Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF01276822
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dc.titleDNA synthesis and mRNA accumulation during germination of embryos of the orchid Spathoglottis plicata
dc.contributor.authorRaghavan, V.
dc.contributor.authorGoh, C.J.
dc.date.accessioned2014-10-27T07:02:57Z
dc.date.available2014-10-27T07:02:57Z
dc.date.issued1994-03
dc.identifier.citationRaghavan, V., Goh, C.J. (1994-03). DNA synthesis and mRNA accumulation during germination of embryos of the orchid Spathoglottis plicata. Protoplasma 183 (1-4) : 137-147. ScholarBank@NUS Repository. https://doi.org/10.1007/BF01276822
dc.identifier.issn0033183X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99666
dc.description.abstractGermination of embryos of the orchid, Spathoglottis plicata Blume involves the formation of a protocorm in which DNA synthesis and cell divisions are confined to the proximal end whereas cells at the distal end undergo enlargement. Although3H-thymidine was incorporated into the distal cells of the embryo during the early period of germination, DNA synthesis was not followed by mitosis and cytokinesis. Poly(A)-RNA detected by in situ hybridization of sections to3H-poly-(U) was present uniformly in all cells of the embryo of the dry seed. However, coincident with the formation of the apical meristem, there was an increase in the density of auto-radiographic silver grains in the cells of the embryo, with a concentration of silver grains in the proximal part. The results indicate that regulatory events in the embryo prior to seed maturity determine the fate of its proximal and distal parts during germination. © 1994 Springer-Verlag.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF01276822
dc.sourceScopus
dc.subjectDNA synthesis
dc.subjectEmbryo development
dc.subjectmRNA accumulation
dc.subjectOrchid embryo
dc.subjectSeed germination
dc.subjectSpathoglottis plicata
dc.typeArticle
dc.contributor.departmentBOTANY
dc.description.doi10.1007/BF01276822
dc.description.sourcetitleProtoplasma
dc.description.volume183
dc.description.issue1-4
dc.description.page137-147
dc.description.codenPROTA
dc.identifier.isiutA1994PZ30300017
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