Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00232785
DC FieldValue
dc.titleAn in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture
dc.contributor.authorLakshmanan, P.
dc.contributor.authorLoh, C.-S.
dc.contributor.authorGoh, C.-J.
dc.date.accessioned2014-10-27T07:02:47Z
dc.date.available2014-10-27T07:02:47Z
dc.date.issued1995-05
dc.identifier.citationLakshmanan, P., Loh, C.-S., Goh, C.-J. (1995-05). An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture. Plant Cell Reports 14 (8) : 510-514. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00232785
dc.identifier.issn07217714
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99649
dc.description.abstractA thin section culture system for rapid regeneration of the monopodial orchid hybrid Aranda Deborah has been developed. Thin sections (0.6-0.7mm thick) obtained by transverse sectioning of a single shoot tip (6-7mm), when cultured in Vacin and Went medium enriched with coconut water (20% v/v), produced an average 13.6 protocorm-like bodies (PLB) after 45 days, compared to 2.7 PLB formed by a single 6-7 mm long shoot tip under same culture condition. Addition of α-naphthaleneacetic acid to Vacin and Went medium enriched with coconut water further increased PLB production by thin sections. PLB developed into plantlets on solid Vacin and Went medium containing 10% (v/v) coconut water and 0.5 g l-1 activated charcoal. With this procedure, more than 80,000 plantlets could be produced from thin sections obtained from a single shoot tip in a year as compared to nearly 11,000 plantlets produced by the conventional shoot tip method. © 1995 Springer-Verlag.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF00232785
dc.sourceScopus
dc.subjectAranda Deborah
dc.subjectorchids
dc.subjectthin section culture
dc.subjecttissue culture
dc.typeArticle
dc.contributor.departmentBOTANY
dc.description.doi10.1007/BF00232785
dc.description.sourcetitlePlant Cell Reports
dc.description.volume14
dc.description.issue8
dc.description.page510-514
dc.description.codenPCRPD
dc.identifier.isiutA1995RK82000009
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.