Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gkr1247
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dc.titleGene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility
dc.contributor.authorLim, C.J.
dc.contributor.authorWhang, Y.R.
dc.contributor.authorKenney, L.J.
dc.contributor.authorYan, J.
dc.date.accessioned2014-10-16T09:26:29Z
dc.date.available2014-10-16T09:26:29Z
dc.date.issued2012-04
dc.identifier.citationLim, C.J., Whang, Y.R., Kenney, L.J., Yan, J. (2012-04). Gene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility. Nucleic Acids Research 40 (8) : 3316-3328. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkr1247
dc.identifier.issn03051048
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/96698
dc.description.abstractNucleoid-associated proteins are bacterial proteins that are responsible for chromosomal DNA compaction and global gene regulation. One such protein is Escherichia coli Histone-like nucleoid structuring protein (H-NS) which functions as a global gene silencer. Whereas the DNA-binding mechanism of H-NS is well-characterized, its paralogue, StpA which is also able to silence genes is less understood. Here we show that StpA is similar to H-NS in that it is able to form a rigid filament along DNA. In contrast to H-NS, the StpA filament interacts with a naked DNA segment to cause DNA bridging which results in simultaneous stiffening and bridging of DNA. DNA accessibility is effectively blocked after the formation of StpA filament on DNA, suggesting rigid filament formation is the important step in promoting gene silencing. We also show that >1mM magnesium promotes higher order DNA condensation, suggesting StpA may also play a role in chromosomal DNA packaging. © 2011 The Author(s).
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPHYSICS
dc.description.doi10.1093/nar/gkr1247
dc.description.sourcetitleNucleic Acids Research
dc.description.volume40
dc.description.issue8
dc.description.page3316-3328
dc.description.codenNARHA
dc.identifier.isiut000303333500011
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