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|Title:||From surface self-assembly to crystallization: Prediction of protein crystallization conditions||Authors:||Jia, Y.
|Issue Date:||6-May-2006||Citation:||Jia, Y., Liu, X.-Y. (2006-05-06). From surface self-assembly to crystallization: Prediction of protein crystallization conditions. Journal of Physical Chemistry B 110 (13) : 6949-6955. ScholarBank@NUS Repository. https://doi.org/10.1021/jp0536089||Abstract:||A new criterion based on surface and volume diffusion kinetics was established to predict protein crystallization. Similar to the layer-by-layer crystal growth process of protein, the kinetics of the two-dimensional self-assembly of protein at the aqueous solution surface provides a convenient and reliable way to estimate the surface integration and the volume transport during protein crystallization. Both the surface and diffusion kinetics were estimated based on the protein self-assembly at the air/solution interface, which can be obtained by measuring the surface tension. A crystallization coefficient is found to provide an effective and reliable criterion to predict protein crystallization conditions. This criterion has been applied to lysozyme, concanavalin A and BSA crystallization, and it turns out to be very successful and more reliable than the second virial coefficient criterion. © 2006 American Chemical Society.||Source Title:||Journal of Physical Chemistry B||URI:||http://scholarbank.nus.edu.sg/handle/10635/96680||ISSN:||15206106||DOI:||10.1021/jp0536089|
|Appears in Collections:||Staff Publications|
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