Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ajpath.2011.03.013
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dc.titleExcessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis
dc.contributor.authorNarasaraju, T.
dc.contributor.authorYang, E.
dc.contributor.authorSamy, R.P.
dc.contributor.authorNg, H.H.
dc.contributor.authorPoh, W.P.
dc.contributor.authorLiew, A.-A.
dc.contributor.authorPhoon, M.C.
dc.contributor.authorVan Rooijen, N.
dc.contributor.authorChow, V.T.
dc.date.accessioned2014-10-16T09:24:40Z
dc.date.available2014-10-16T09:24:40Z
dc.date.issued2011-07
dc.identifier.citationNarasaraju, T., Yang, E., Samy, R.P., Ng, H.H., Poh, W.P., Liew, A.-A., Phoon, M.C., Van Rooijen, N., Chow, V.T. (2011-07). Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis. American Journal of Pathology 179 (1) : 199-210. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ajpath.2011.03.013
dc.identifier.issn00029440
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/96542
dc.description.abstractComplications of acute respiratory distress syndrome (ARDS) are common among critically ill patients infected with highly pathogenic influenza viruses. Macrophages and neutrophils constitute the majority of cells recruited into infected lungs, and are associated with immunopathology in influenza pneumonia. We examined pathological manifestations in models of macrophage- or neutrophil-depleted mice challenged with sublethal doses of influenza A virus H1N1 strain PR8. Infected mice depleted of macrophages displayed excessive neutrophilic infiltration, alveolar damage, and increased viral load, later progressing into ARDS-like pathological signs with diffuse alveolar damage, pulmonary edema, hemorrhage, and hypoxemia. In contrast, neutrophil-depleted animals showed mild pathology in lungs. The brochoalveolar lavage fluid of infected macrophage-depleted mice exhibited elevated protein content, T1-α, thrombomodulin, matrix metalloproteinase-9, and myeloperoxidase activities indicating augmented alveolar-capillary damage, compared to neutrophil-depleted animals. We provide evidence for the formation of neutrophil extracellular traps (NETs), entangled with alveoli in areas of tissue injury, suggesting their potential link with lung damage. When co-incubated with infected alveolar epithelial cells in vitro, neutrophils from infected lungs strongly induced NETs generation, and augmented endothelial damage. NETs induction was abrogated by anti-myeloperoxidase antibody and an inhibitor of superoxide dismutase, thus implying that NETs generation is induced by redox enzymes in influenza pneumonia. These findings support the pathogenic effects of excessive neutrophils in acute lung injury of influenza pneumonia by instigating alveolar-capillary damage. © 2011 American Society for Investigative Pathology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ajpath.2011.03.013
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPHYSICS
dc.description.doi10.1016/j.ajpath.2011.03.013
dc.description.sourcetitleAmerican Journal of Pathology
dc.description.volume179
dc.description.issue1
dc.description.page199-210
dc.description.codenAJPAA
dc.identifier.isiut000298307100021
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