Please use this identifier to cite or link to this item: https://doi.org/10.1071/CH09112
DC FieldValue
dc.titleImmobilized whole cells as effective catalysts for chiral alcohol production
dc.contributor.authorNg, J.F.
dc.contributor.authorJaenicke, S.
dc.date.accessioned2014-10-16T08:48:13Z
dc.date.available2014-10-16T08:48:13Z
dc.date.issued2009
dc.identifier.citationNg, J.F., Jaenicke, S. (2009). Immobilized whole cells as effective catalysts for chiral alcohol production. Australian Journal of Chemistry 62 (9) : 1034-1039. ScholarBank@NUS Repository. https://doi.org/10.1071/CH09112
dc.identifier.issn00049425
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/95469
dc.description.abstractRecombinant Escherichia coli overexpressing the gene LbADH, which encodes for an alcohol dehydrogenase from Lactobacillus brevis, was successfully transformed and cultured. The cells are able to catalyze the reduction of pro-chiral ketones, e.g. ethyl acetoacetate into R-()ethyl hydroxybutyrate (EHB) with high conversion and enantiomeric excess 99%. Immobilizing the whole cells in alginate beads leads to a catalyst with improved stability and ease of handling while maintaining the high activity of the free cells. The whole-cell catalyst was tested in a stirred batch reactor (CSTR) and in a continuously operated packed-bed reactor. An Mg2+ concentration of 2 mM was crucial for maintaining the activity of the biocatalyst. After a partial optimization of the process conditions, a productivity of 1.4 gEHB gwcw -1 h-1 could be maintained in a continuous flow reactor over a prolonged period of time. © CSIRO 2009.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1071/CH09112
dc.sourceScopus
dc.typeConference Paper
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1071/CH09112
dc.description.sourcetitleAustralian Journal of Chemistry
dc.description.volume62
dc.description.issue9
dc.description.page1034-1039
dc.description.codenAJCHA
dc.identifier.isiut000269853200014
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