Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2007.08.013
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dc.titleThe self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection
dc.contributor.authorWang, Y.
dc.contributor.authorKe, C.-Y.
dc.contributor.authorWeijie Beh, C.
dc.contributor.authorLiu, S.-Q.
dc.contributor.authorGoh, S.-H.
dc.contributor.authorYang, Y.-Y.
dc.date.accessioned2014-10-16T08:45:47Z
dc.date.available2014-10-16T08:45:47Z
dc.date.issued2007-12
dc.identifier.citationWang, Y., Ke, C.-Y., Weijie Beh, C., Liu, S.-Q., Goh, S.-H., Yang, Y.-Y. (2007-12). The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection. Biomaterials 28 (35) : 5358-5368. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2007.08.013
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/95264
dc.description.abstractCationic micelles self-assembled from a biodegradable amphiphilic copolymer, poly{(N-methyldietheneamine sebacate)-co-[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate} (P(MDS-co-CES)) have recently been reported for efficient gene delivery and co-delivery of drug and nucleic acid. In this study, poly(ethylene glycol) (PEG) of various molecular weights (Mn=550, 1100 and 2000) was conjugated to P(MDS-co-CES) having different cholesterol grafting degrees to improve the stability of micelle/DNA complexes in the blood for systemic in vivo gene delivery. DNA binding ability, gene transfection efficiency and cytotoxicity of P(MDS-co-CES), PMDS, PEGylated PMDS and PEGylated P(MDS-co-CES) micelles were studied and compared. As with P(MDS-co-CES), PEG-P(MDS-co-CES) polymers could also self-assemble into stable micelles of small size. However, PMDS and PEG-PMDS without cholesterol could not form stable micelles but formed large particles. PEGylation of polymers significantly decreased their gene transfection efficiency in HEK293, HepG2, HeLa, MDA-MB-231 and 4T1 cells. However, increasing N/P ratio promoted gene transfection. An increased cholesterol grafting degree led to greater gene expression level possibly because of the more stable core-shell structure of the micelles. PEG550-P(MDS-co-CES) micelles induced high gene transfection level, comparable to that provided by P(MDS-co-CES) micelles. PEGylated polymers were much less cytotoxic than P(MDS-co-CES). PEGylated P(MDS-co-CES) micelles may provide a promising non-viral vector for systemic in vivo gene delivery. © 2007 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2007.08.013
dc.sourceScopus
dc.subjectCationic polymer micelles
dc.subjectGene transfection
dc.subjectPEGylation
dc.subjectSelf-assembly
dc.typeArticle
dc.contributor.departmentPHARMACY
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1016/j.biomaterials.2007.08.013
dc.description.sourcetitleBiomaterials
dc.description.volume28
dc.description.issue35
dc.description.page5358-5368
dc.description.codenBIMAD
dc.identifier.isiut000250860000015
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