Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.biomaterials.2007.08.013
DC Field | Value | |
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dc.title | The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection | |
dc.contributor.author | Wang, Y. | |
dc.contributor.author | Ke, C.-Y. | |
dc.contributor.author | Weijie Beh, C. | |
dc.contributor.author | Liu, S.-Q. | |
dc.contributor.author | Goh, S.-H. | |
dc.contributor.author | Yang, Y.-Y. | |
dc.date.accessioned | 2014-10-16T08:45:47Z | |
dc.date.available | 2014-10-16T08:45:47Z | |
dc.date.issued | 2007-12 | |
dc.identifier.citation | Wang, Y., Ke, C.-Y., Weijie Beh, C., Liu, S.-Q., Goh, S.-H., Yang, Y.-Y. (2007-12). The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection. Biomaterials 28 (35) : 5358-5368. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2007.08.013 | |
dc.identifier.issn | 01429612 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/95264 | |
dc.description.abstract | Cationic micelles self-assembled from a biodegradable amphiphilic copolymer, poly{(N-methyldietheneamine sebacate)-co-[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate} (P(MDS-co-CES)) have recently been reported for efficient gene delivery and co-delivery of drug and nucleic acid. In this study, poly(ethylene glycol) (PEG) of various molecular weights (Mn=550, 1100 and 2000) was conjugated to P(MDS-co-CES) having different cholesterol grafting degrees to improve the stability of micelle/DNA complexes in the blood for systemic in vivo gene delivery. DNA binding ability, gene transfection efficiency and cytotoxicity of P(MDS-co-CES), PMDS, PEGylated PMDS and PEGylated P(MDS-co-CES) micelles were studied and compared. As with P(MDS-co-CES), PEG-P(MDS-co-CES) polymers could also self-assemble into stable micelles of small size. However, PMDS and PEG-PMDS without cholesterol could not form stable micelles but formed large particles. PEGylation of polymers significantly decreased their gene transfection efficiency in HEK293, HepG2, HeLa, MDA-MB-231 and 4T1 cells. However, increasing N/P ratio promoted gene transfection. An increased cholesterol grafting degree led to greater gene expression level possibly because of the more stable core-shell structure of the micelles. PEG550-P(MDS-co-CES) micelles induced high gene transfection level, comparable to that provided by P(MDS-co-CES) micelles. PEGylated polymers were much less cytotoxic than P(MDS-co-CES). PEGylated P(MDS-co-CES) micelles may provide a promising non-viral vector for systemic in vivo gene delivery. © 2007 Elsevier Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2007.08.013 | |
dc.source | Scopus | |
dc.subject | Cationic polymer micelles | |
dc.subject | Gene transfection | |
dc.subject | PEGylation | |
dc.subject | Self-assembly | |
dc.type | Article | |
dc.contributor.department | PHARMACY | |
dc.contributor.department | CHEMISTRY | |
dc.description.doi | 10.1016/j.biomaterials.2007.08.013 | |
dc.description.sourcetitle | Biomaterials | |
dc.description.volume | 28 | |
dc.description.issue | 35 | |
dc.description.page | 5358-5368 | |
dc.description.coden | BIMAD | |
dc.identifier.isiut | 000250860000015 | |
Appears in Collections: | Staff Publications |
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