Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00323979
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dc.titleFluorescence detection in capillary electrophoresis using a variable wavelength epi-fluorescence microscope
dc.contributor.authorChan, K.T.
dc.contributor.authorYao, Y.J.
dc.contributor.authorNg, C.L.
dc.contributor.authorLi, S.F.Y.
dc.date.accessioned2014-10-16T08:28:48Z
dc.date.available2014-10-16T08:28:48Z
dc.date.issued1994-07
dc.identifier.citationChan, K.T., Yao, Y.J., Ng, C.L., Li, S.F.Y. (1994-07). Fluorescence detection in capillary electrophoresis using a variable wavelength epi-fluorescence microscope. Fresenius' Journal of Analytical Chemistry 349 (7) : 487-490. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00323979
dc.identifier.issn09370633
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/93821
dc.description.abstractA capillary electrophoresis fluorescence detector is described. A high-pressure mercury lamp with a filter block allowed the selection of a particular excitation waveband. Detection was performed on-column, the fluorescence emission was monitored and measured with a silicon photodiode detector with a built-in amplifier. The concentration limit of detection (CLOD) of 0.4 ng/mL was obtained for rhodamine B, a fluorescent indicator. Based on an estimated injection volume of 2.5 nL, the mass limit of detection (MLOD) was 2.1×10-18 mol. The separation of three fluorescent indicators: thionine, eosin yellowish and rhodamine B, was achieved in less than 6 min. The separation of nine porphyrin-free acids using the system developed was also demonstrated. The advantages and potential of using an epi-illumination microscope as a versatile and sensitive fluorescence detection system for capillary electrophoresis are described. © 1994 Springer-Verlag.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF00323979
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1007/BF00323979
dc.description.sourcetitleFresenius' Journal of Analytical Chemistry
dc.description.volume349
dc.description.issue7
dc.description.page487-490
dc.description.codenFJACE
dc.identifier.isiutA1994NW29800002
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