Please use this identifier to cite or link to this item: https://doi.org/10.1002/elps.200406018
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dc.titleDetermination of the bacterial pathogen Edwardsiella tarda in fish species by capillary electrophoresis with blue light-emitting diode-induced fluorescence
dc.contributor.authorYu, L.
dc.contributor.authorYuan, L.
dc.contributor.authorFeng, H.
dc.contributor.authorLi, S.F.Y.
dc.date.accessioned2014-10-16T08:25:43Z
dc.date.available2014-10-16T08:25:43Z
dc.date.issued2004-10
dc.identifier.citationYu, L., Yuan, L., Feng, H., Li, S.F.Y. (2004-10). Determination of the bacterial pathogen Edwardsiella tarda in fish species by capillary electrophoresis with blue light-emitting diode-induced fluorescence. Electrophoresis 25 (18-19) : 3139-3144. ScholarBank@NUS Repository. https://doi.org/10.1002/elps.200406018
dc.identifier.issn01730835
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/93560
dc.description.abstractHigh-performance capillary electrophoresis (HPCE) has been applied to the identification, separation, and quantitation of intact bacteria. We demonstrate that a pathogen (Edwardsiella tarda) which causes systemic infection in commercially important fish species can be rapidly identified and determined ( 1 200 000 theoretical plates/m) was achieved with this rapid and efficient CE method. It was found that proper sample vortexing (90 s) would be beneficial to disperse aggregated cells and facilitate the focusing of intact cells during electrophoresis. Ionization of the surface constituents of Edwardsiella tarda cells provided efficient surface charges for the intact cells to be separated from the EOF and damaged or lysed cells when the separation was performed in running buffer (3.94 mM Tris, 0.56 mM borate, 0.013 mM EDTA) at pH 10.5. The limit of detection (LOD) and recovery were found to be 4.2 × 104 cells/mL and 70.0%, respectively. This proposed CE method could become an effective tool for diagnosis and tracking of certain diseases caused by bacteria in fish species as well as in human beings. © 2004 Wiley-VCH Verlag GmbH & Co. KGaA.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/elps.200406018
dc.sourceScopus
dc.subjectBacteria
dc.subjectBlue light-emitting diode-induced fluorescence
dc.subjectCapillary electrophoresis
dc.subjectEdwardsiella tarda
dc.subjectSYTO 13 green nucleic acid stain
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1002/elps.200406018
dc.description.sourcetitleElectrophoresis
dc.description.volume25
dc.description.issue18-19
dc.description.page3139-3144
dc.description.codenELCTD
dc.identifier.isiut000224649500013
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