Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0008-6215(01)00084-2
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dc.titleConcurrent production of chitin from shrimp shells and fungi
dc.contributor.authorTeng, W.L.
dc.contributor.authorKhor, E.
dc.contributor.authorTan, T.K.
dc.contributor.authorLim, L.Y.
dc.contributor.authorTan, S.C.
dc.date.accessioned2014-10-16T08:23:21Z
dc.date.available2014-10-16T08:23:21Z
dc.date.issued2001-06-04
dc.identifier.citationTeng, W.L., Khor, E., Tan, T.K., Lim, L.Y., Tan, S.C. (2001-06-04). Concurrent production of chitin from shrimp shells and fungi. Carbohydrate Research 332 (3) : 305-316. ScholarBank@NUS Repository. https://doi.org/10.1016/S0008-6215(01)00084-2
dc.identifier.issn00086215
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/93350
dc.description.abstractCrustacean shells constitute the traditional and current commercial source of chitin. Conversely, the control of fungal fermentation processes to produce quality chitin makes fungal mycelia an attractive alternative source. Therefore, the exploitation of both of these sources to produce chitin in a concurrent process should be advantageous and is reported here. Three proteolytic Aspergillus niger (strains 0576, 0307 and 0474) were selected from a screening for protease activity from among 34 zygomycete and deuteromycete strains. When fungi and shrimp shell powder were combined in a single reactor, the release of protease by the fungi facilitated the deproteinization of shrimp-shell powder and the release of hydrolyzed proteins. The hydrolyzed proteins in turn were utilized as a nitrogen source for fungal growth, leading to a lowering of the pH of the fermentation medium, thereby further enhancing the demineralization of the shrimp-shell powder. The shrimp-shell powders and fungal mycelia were separated after fermentation and extracted for chitin with 5% LiCl/DMAc solvent. Chitin isolates from the shells were found to have a protein content of less than 5%, while chitin isolates from the three fungal mycelia strains had protein content in the range of 10-15%. The relative molecular weights as estimated by GPC for all chitin samples were in the 105 dalton range. All samples displayed characteristic profiles for chitin in their FTIR and solid-state NMR spectra. All chitin samples evaluated with MTT and Neutral Red assays with three commercial cell lines did not display cytotoxic effects. © 2001 Elsevier Science Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0008-6215(01)00084-2
dc.sourceScopus
dc.subjectChitin
dc.subjectCytotoxicity
dc.subjectFermentation
dc.subjectFungi
dc.subjectShrimp shells
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentPHARMACY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0008-6215(01)00084-2
dc.description.sourcetitleCarbohydrate Research
dc.description.volume332
dc.description.issue3
dc.description.page305-316
dc.description.codenCRBRA
dc.identifier.isiut000169024600007
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