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Title: An ISFET-based immunosensor for the detection of β-Bungarotoxin
Authors: Selvanayagam, Z.E. 
Neuzil, P.
Gopalakrishnakone, P. 
Sridhar, U.
Singh, M. 
Ho, L.C.
Keywords: β-Bungarotoxin
Diagnosis of snake envenomation
Ion-sensitive field effect transistor (ISFET)
Issue Date: Sep-2002
Citation: Selvanayagam, Z.E., Neuzil, P., Gopalakrishnakone, P., Sridhar, U., Singh, M., Ho, L.C. (2002-09). An ISFET-based immunosensor for the detection of β-Bungarotoxin. Biosensors and Bioelectronics 17 (9) : 821-826. ScholarBank@NUS Repository.
Abstract: An ion-sensitive field effect transistor (ISFET)-based immunosensor was developed to detect/quantitate β-Bungarotoxin (β-BuTx), a potent presynaptic neurotoxin from the venom of Bungarus multicinctus. A murine monoclonal antibody (mAb 15) specific to β-BuTx was immobilized onto silicon nitride wafers after silanization and activation with glutaraldehyde. A chip based enzyme linked-immunosorbantassay (ELISA) was performed to ascertain antigen binding to the immobilized antibody. To develop an electrochemical immunosensing system for the detection/quantitation of β-BuTx, an ISFET was used as a solid phase detector. MAb 15 was immobilized on the gate region of the ISFET. The antigen antibody reaction was monitored by the addition of urease conjugated rabbit anti-β-BuTx antibodies. The sensor can detect toxin level as low as 15.6 ng/ml. The efficacy of the sensor for the determination of β-BuTx from B. multicinctus venom was demonstrated in mouse model. Toxin concentration was highest at the site of injection (748.0±26 ng/ml) and moderate amount was found in the plasma (158.5±13 ng/ml). © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Biosensors and Bioelectronics
ISSN: 09565663
DOI: 10.1016/S0956-5663(02)00075-1
Appears in Collections:Staff Publications

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