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Title: Alginate-chitosan coacervation in production of artificial seeds
Authors: Tay, L.-F.
Khoh, L.-K.
Loh, C.-S. 
Khor, E. 
Keywords: Artificial seed
Complex coacervation
Embryoid viability
Sodium alginate
Issue Date: 5-Aug-1993
Citation: Tay, L.-F.,Khoh, L.-K.,Loh, C.-S.,Khor, E. (1993-08-05). Alginate-chitosan coacervation in production of artificial seeds. Biotechnology and Bioengineering 42 (4) : 449-454. ScholarBank@NUS Repository.
Abstract: Survival of secondary embryoids of winter oilseed rape (Brassica napus ssp. oleifera cv. Primor) has been used as an assay for the development of artificial seeds involving complex coacervation of alginate (polyanion) with chitosan (polycation). Germination frequency of 100% was achieved for encapuslated embryoids when alginate formed the inner matrix and chitosan the outer layer. When the matrix makeup was reversed, there was no germination of embryoids. The artificial seeds produced were hardened in dilute alkaline solutions of NaOH and Ca(OH)2. An optimum setting time could be selected based on a quantitative measurement of resistance of hardened capsuless to compression and the germination frequency of the encapsulated embryoids.
Source Title: Biotechnology and Bioengineering
ISSN: 00063592
Appears in Collections:Staff Publications

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