Please use this identifier to cite or link to this item: https://doi.org/10.1021/ac900793w
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dc.titleLiquid crystal multiplexed protease assays reporting enzymatic activities as optical bar charts
dc.contributor.authorBi, X.
dc.contributor.authorSiok, L.L.
dc.contributor.authorYang, K.-L.
dc.date.accessioned2014-10-09T06:52:36Z
dc.date.available2014-10-09T06:52:36Z
dc.date.issued2009-07-01
dc.identifier.citationBi, X., Siok, L.L., Yang, K.-L. (2009-07-01). Liquid crystal multiplexed protease assays reporting enzymatic activities as optical bar charts. Analytical Chemistry 81 (13) : 5503-5509. ScholarBank@NUS Repository. https://doi.org/10.1021/ac900793w
dc.identifier.issn00032700
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/89337
dc.description.abstractWe describe a highly sensitive, substrate-specific, label-free, and multiplexed protease assay which reports proteases activities as an optical bar chart, allowing test results to be easily assessed by laymen with the naked eye. First, an oligopeptide microarray having six rows of immobilized oligopeptides, with well-controlled orientations and concentration gradients, is immersed in a buffer solution containing proteases. Then, a thin layer liquid crystal is supported on the microarray to transduce the oligopeptide cleavage event into an optical bar chart of different colors and lengths. This type of optical bar chart provides very rich information such as protease concentration, incubation time, surface densities of oligopeptides, etc. Both trypsin and chymotrypsin can be detected by using this assay within 3 h. The capability of the multiplexed protease assay opens up possibilities for detecting toxins such as botulinum neurotoxins which are known to cleave proteins and affect the docking and fusing synaptic vesicles. © 2009 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/ac900793w
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1021/ac900793w
dc.description.sourcetitleAnalytical Chemistry
dc.description.volume81
dc.description.issue13
dc.description.page5503-5509
dc.description.codenANCHA
dc.identifier.isiut000267609500054
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