Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.enzmictec.2005.11.048
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dc.titleGlobal transcriptional analysis of metabolic burden due to plasmid maintenance in Escherichia coli DH5α during batch fermentation
dc.contributor.authorOw, D.S.-W.
dc.contributor.authorNissom, P.M.
dc.contributor.authorPhilp, R.
dc.contributor.authorOh, S.K.-W.
dc.contributor.authorYap, M.G.-S.
dc.date.accessioned2014-10-09T06:48:56Z
dc.date.available2014-10-09T06:48:56Z
dc.date.issued2006-07-03
dc.identifier.citationOw, D.S.-W., Nissom, P.M., Philp, R., Oh, S.K.-W., Yap, M.G.-S. (2006-07-03). Global transcriptional analysis of metabolic burden due to plasmid maintenance in Escherichia coli DH5α during batch fermentation. Enzyme and Microbial Technology 39 (3) : 391-398. ScholarBank@NUS Repository. https://doi.org/10.1016/j.enzmictec.2005.11.048
dc.identifier.issn01410229
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/89012
dc.description.abstractDNA plasmids of Escherichia coli are common vectors for recombinant protein and metabolite production and have potential therapeutic applications as genetic vaccines and therapeutics. However, plasmid maintenance imposes a metabolic burden on the host cells, resulting in reduced growth rate and cell density. In 2 L batch fermentation, DH5α cells carrying a 7.3 kb NS3 plasmid had a lower specific growth rate than the non-plasmid-bearing host (0.64 h-1 versus 0.87 h-1). In this work, global transcriptional analysis was combined with proteomics studies to evaluate the effect of plasmid maintenance on gene expression. Global transcriptional expression analysis of plasmid-bearing cells over host showed a general trend of downregulated biosynthetic/energy metabolism genes, differentially expressed transport genes and upregulated heat shock proteins. In the central metabolic pathways, most glycolytic genes were downregulated, while less expression difference was found in the pentose phosphate pathway. Expression ratios of 19 proteins identified from proteomics studies were consistent with these observations. Our findings suggest that plasmid maintenance alone perturbs global gene regulation, and leads to significant changes in central metabolic pathways in the host. This work contributes to our understanding of plasmid metabolic load at the gene expression level and could potentially aid in future metabolic engineering efforts. © 2006 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.enzmictec.2005.11.048
dc.sourceScopus
dc.subjectDH5α
dc.subjectFermentation
dc.subjectMetabolic burden
dc.subjectPlasmid maintenance
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.enzmictec.2005.11.048
dc.description.sourcetitleEnzyme and Microbial Technology
dc.description.volume39
dc.description.issue3
dc.description.page391-398
dc.description.codenEMTED
dc.identifier.isiut000238311900009
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