Collagen-coupled poly(2-hydroxyethyl methacrylate)-Si(111) hybrid surfaces for cell immobilization

Abstract

To improve the biocompatibility of silicon-based implantable micro- and nanodevices, and to tailor silicon surfaces for controlled cell immobilization, well-defined functional polymer-Si(111) hybrids, consisting of nearly monodispersed poly(2-hydroxyethyl methacrylate [P(HEMA)] with covalently coupled collagen and tethered (Si-C bonded) on the silicon surfaces, were prepared. HEMA was graft polymerized on the hydrogen-terminated Si(111) surface (Si-H surface) via surface-initiated atom transfer radical polymerization (ATRP) to give rise to the Si-g-P(HEMA) hybrid. The active chloride end groups preserved throughout the ATRP process and the chloride groups converted from some (∼20%) of the OH groups of the P(HEMA) brushes were used as the leaving groups for nucleopliilic reaction with the -NH2 groups of collagen to give rise to the Si-g-P(HEMA)-collagen surface conjugates. These hybrid surfaces were evaluated by culturing 3T3 fibroblasts. The biocompatible Si-g-P(HEMA) hybrid surface resisted attachment and growth of this cell line. The Si-g-P(HEMA)-collagen hybrid surfaces, on the other hand, exhibited good cell adhesion and growth characteristics, and the extent of cell immobilization could be controlled by adjusting the amount of immobilized collagen. Thus, incorporating the collagen-coupled P(HEMA) onto silicon surfaces via robust Si-C bonds may endow the silicon substrates with new and interesting properties for potential applications in silicon-based implantable devices, such as molecular sensors and biochips. © Mary Ann Liebert, Inc.