Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bpc.2003.10.021
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dc.titleCloud-point temperature and liquid-liquid phase separation of supersaturated lysozyme solution
dc.contributor.authorLu, J.
dc.contributor.authorCarpenter, K.
dc.contributor.authorLi, R.-J.
dc.contributor.authorWang, X.-J.
dc.contributor.authorChing, C.-B.
dc.date.accessioned2014-10-09T06:44:48Z
dc.date.available2014-10-09T06:44:48Z
dc.date.issued2004-04-01
dc.identifier.citationLu, J., Carpenter, K., Li, R.-J., Wang, X.-J., Ching, C.-B. (2004-04-01). Cloud-point temperature and liquid-liquid phase separation of supersaturated lysozyme solution. Biophysical Chemistry 109 (1) : 105-112. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bpc.2003.10.021
dc.identifier.issn03014622
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/88654
dc.description.abstractThe detailed understanding of the structure of biological macromolecules reveals their functions, and is thus important in the design of new medicines and for engineering molecules with improved properties for industrial applications. Although techniques used for protein crystallization have been progressing greatly, protein crystallization may still be considered an art rather than a science, and successful crystallization remains largely empirical and operator-dependent. In this work, a microcalorimetric technique has been utilized to investigate liquid-liquid phase separation through measuring cloud-point temperature Tcloud for supersaturated lysozyme solution. The effects of ionic strength and glycerol on the cloud-point temperature are studied in detail. Over the entire range of salt concentrations studied, the cloud-point temperature increases monotonically with the concentration of sodium chloride. When glycerol is added as additive, the solubility of lysozyme is increased, whereas the cloud-point temperature is decreased. © 2003 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bpc.2003.10.021
dc.sourceScopus
dc.subjectBiocrystallization
dc.subjectCloud-point temperature
dc.subjectLiquid-liquid phase separation
dc.subjectMicrocalorimetry
dc.typeArticle
dc.contributor.departmentCHEMICAL AND PROCESS ENGINEERING CENTRE
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.bpc.2003.10.021
dc.description.sourcetitleBiophysical Chemistry
dc.description.volume109
dc.description.issue1
dc.description.page105-112
dc.description.codenBICIA
dc.identifier.isiut000220811700008
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