Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2008.11.021
DC FieldValue
dc.titleA liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors
dc.contributor.authorHartono, D.
dc.contributor.authorLai, S.L.
dc.contributor.authorYang, K.-L.
dc.contributor.authorYung, L.-Y.L.
dc.date.accessioned2014-10-09T06:42:32Z
dc.date.available2014-10-09T06:42:32Z
dc.date.issued2009-03-15
dc.identifier.citationHartono, D., Lai, S.L., Yang, K.-L., Yung, L.-Y.L. (2009-03-15). A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors. Biosensors and Bioelectronics 24 (7) : 2289-2293. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2008.11.021
dc.identifier.issn09565663
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/88456
dc.description.abstractWe report a liquid crystal (LC)-based sensor for real-time and label-free identification of phospholipase-like toxins. Beta-bungarotoxin exhibits Ca2+-dependent phospholipase A2 activity whereas alpha-bungarotoxin and myotoxin II do not exhibit any phospholipase activity. The sensor can selectively identify beta-bungarotoxin, when it hydrolyzes a phospholipid monolayer self-assembled at aqueous-LC interface, through orientational responses of LCs. As a result, optical signals that reflect the spatial and temporal distribution of phospholipids during the hydrolysis can therefore be generated in a real-time manner. The sensor is very sensitive and requires less than 5 pg of beta-bungarotoxin for the detection. When phospholipase A2 inhibitors are introduced together with beta-bungarotoxin, no orientational response of LCs can be observed. In addition, the regeneration of the sensor can be done without affecting the sensing performance. This work demonstrates a simple and cost-effective LC-based sensor for identifying phospholipase-like toxins and for screening compound libraries to find potential toxin inhibitors. © 2008 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bios.2008.11.021
dc.sourceScopus
dc.subjectBeta-bungarotoxin
dc.subjectLiquid crystal
dc.subjectPhospholipase
dc.subjectReal-time detection
dc.subjectToxin inhibitor
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.bios.2008.11.021
dc.description.sourcetitleBiosensors and Bioelectronics
dc.description.volume24
dc.description.issue7
dc.description.page2289-2293
dc.description.codenBBIOE
dc.identifier.isiut000264791000080
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

62
checked on May 20, 2022

WEB OF SCIENCETM
Citations

60
checked on May 20, 2022

Page view(s)

109
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.