Please use this identifier to cite or link to this item: https://doi.org/10.3892/ijmm.2013.1487
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dc.titleTroglitazone upregulates PTEN expression and induces the apoptosis of pulmonary artery smooth muscle cells under hypoxic conditions
dc.contributor.authorPi, W.-F.
dc.contributor.authorGuo, X.-J.
dc.contributor.authorSu, L.-P.
dc.contributor.authorXu, W.-G.
dc.date.accessioned2014-10-08T09:48:37Z
dc.date.available2014-10-08T09:48:37Z
dc.date.issued2013-11
dc.identifier.citationPi, W.-F., Guo, X.-J., Su, L.-P., Xu, W.-G. (2013-11). Troglitazone upregulates PTEN expression and induces the apoptosis of pulmonary artery smooth muscle cells under hypoxic conditions. International Journal of Molecular Medicine 32 (5) : 1101-1109. ScholarBank@NUS Repository. https://doi.org/10.3892/ijmm.2013.1487
dc.identifier.issn11073756
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/88195
dc.description.abstractThe increased proliferation and decreased apop-tosis of pulmonary artery smooth muscle cells (PASMCs) are the main causes of hypoxic pulmonary hypertension. In this study, we investigated the role of troglitazone [peroxisome proliferator-activated receptor γ (PPARγ) agonist] in the regulation of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) expression and the apoptosis of PASMCs under hypoxic conditions. Normal human PASMCs were cultured in growth medium (GM) and treated with troglitazone (0.5-80 μM) under hypoxic conditions (5% CO2+94% N2+1% O2) for 72 h. The gene expression of PTEN, AKT-1 and AKT-2 was determined by quantitative reverse transcription PCR (qRT-PCR). The protein expression level of PTEN, AKT and phosphorylated AKT (p-AKT) was determined by western blot analysis. The apoptosis of PASMCs was determined by measuring the activities of caspase-3, -8 and -9 and by TUNEL assay. The proliferation rate of the PASMCs was altered in a concentration-dependent manner by troglitazone. A signifi-cantly reduced proliferation rate was observed at troglitazone concentrations starting from 20 μM under hypoxic conditions (72±5.8%). Although the gene expression levels of PTEN were increased, the gene expression levels of AKT-1 and AKT-2 remained unaltered. Consistent with this, PTEN protein expression was also altered in a concentration-dependent manner by troglitazone. Although AKT expression was unaltered in all the cell samples, reduced p-AKT expression was observed in the troglitazone-treated PASMCs. Troglitazone increased the activities of caspase-3, -8 and -9 in the PASMCs. bpV(HOpic) (PTEN inhibitor) and GW9662 (PPARγ inhibitor) inhibited PTEN protein expression and recovered the proliferation rate of the PASMCs. TUNEL assay demonstrated that troglitazone significantly increased the apoptosis of PASMCs under hypoxic conditions. In conclusion, troglitazone increases PTEN expression under hypoxic conditions in a concentration-dependent manner. Troglitazone increases the apoptosis of PASMCs under hypoxic conditions. The increase in PTEN expression is mediated through the PPARγ signaling pathway.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.3892/ijmm.2013.1487
dc.sourceScopus
dc.subjectApoptosis
dc.subjectPeroxisome proliferator-activated receptor γ
dc.subjectProliferation
dc.subjectPulmonary artery hypertension
dc.subjectSmooth muscle cells
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.3892/ijmm.2013.1487
dc.description.sourcetitleInternational Journal of Molecular Medicine
dc.description.volume32
dc.description.issue5
dc.description.page1101-1109
dc.description.codenIJMMF
dc.identifier.isiut000324982500016
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