Please use this identifier to cite or link to this item: https://doi.org/10.1038/ismej.2010.27
DC FieldValue
dc.titleIdentification and transcriptional analysis of trans-DCE-producing reductive dehalogenases in Dehalococcoides species
dc.contributor.authorChow, W.L.
dc.contributor.authorCheng, D.
dc.contributor.authorWang, S.
dc.contributor.authorHe, J.
dc.date.accessioned2014-10-08T08:32:31Z
dc.date.available2014-10-08T08:32:31Z
dc.date.issued2010-08
dc.identifier.citationChow, W.L., Cheng, D., Wang, S., He, J. (2010-08). Identification and transcriptional analysis of trans-DCE-producing reductive dehalogenases in Dehalococcoides species. ISME Journal 4 (8) : 1020-1030. ScholarBank@NUS Repository. https://doi.org/10.1038/ismej.2010.27
dc.identifier.issn17517362
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/87522
dc.description.abstractDuring microbial reductive dechlorination of tetrachloroethene (PCE) and trichloroethene (TCE), trans-1, 2-dichloroethene (trans-DCE) has been observed to be produced predominantly by certain mixed and pure cultures. However, the reductive dehalogenase (RDase) genes involved in trans-DCE generation remain elusive. In this study, identification and transcriptional analysis of RDases were conducted on trans-DCE-producing Dehalococcoides sp. strain MB. Two pairs of degenerate primers targeting the conserved regions of RDases in known Dehalococcoides species were applied to amplify the putative RDase genes of strain MB. Cloning and restriction analysis revealed the presence of seven unique RDase gene fragments (dceA1 to dceA7) that possess sequence identity to known RDase genes. Gene expression analysis of the PCE-grown culture MB exhibited 10-fold regulation of the RDase gene dceA6 (designated mbrA gene), suggesting that it is involved in the production of trans-DCE. This is in agreement with the molecular size of the most abundant protein that is resolved on the denaturing protein gel. Complete sequence of the mbrA gene was obtained by chromosome walking, and the upstream of it is a regulator of transcription, indicating that the expression of this functional gene is tightly controlled in the microbe. The mbrA gene was subsequently found to be present in other trans-DCE-producing cultures containing Dehalococcoides sp. The new mbrA gene identified in this study may serve as an important biomarker for evaluating, predicting and elucidating the biological production of trans-DCE in the chloroethene-contaminated sites. © 2010 International Society for Microbial Ecology All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/ismej.2010.27
dc.sourceScopus
dc.subjectbioremediation
dc.subjectchloroethenes
dc.subjectdehalococcoides
dc.subjectRDases
dc.subjecttrans-DCE
dc.typeArticle
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.description.doi10.1038/ismej.2010.27
dc.description.sourcetitleISME Journal
dc.description.volume4
dc.description.issue8
dc.description.page1020-1030
dc.identifier.isiut000280592600006
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

53
checked on Apr 3, 2020

WEB OF SCIENCETM
Citations

49
checked on Mar 18, 2020

Page view(s)

49
checked on Mar 29, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.