Please use this identifier to cite or link to this item: https://doi.org/10.1042/BA20040066
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dc.titleA detailed understanding of the enhanced hypothermic productivity of interferon-γ by Chinese-hamster ovary cells
dc.contributor.authorFox, S.R.
dc.contributor.authorTan, H.K.
dc.contributor.authorTan, M.C.
dc.contributor.authorWong, S.C.N.C.
dc.contributor.authorYap, M.G.S.
dc.contributor.authorWang, D.I.C.
dc.date.accessioned2014-10-07T09:47:31Z
dc.date.available2014-10-07T09:47:31Z
dc.date.issued2005-06
dc.identifier.citationFox, S.R., Tan, H.K., Tan, M.C., Wong, S.C.N.C., Yap, M.G.S., Wang, D.I.C. (2005-06). A detailed understanding of the enhanced hypothermic productivity of interferon-γ by Chinese-hamster ovary cells. Biotechnology and Applied Biochemistry 41 (3) : 255-264. ScholarBank@NUS Repository. https://doi.org/10.1042/BA20040066
dc.identifier.issn08854513
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/86166
dc.description.abstractCulturing CHO (Chinese-hamster ovary) cells at low temperature leads to growth arrest in the G0/G1 phase of the cell cycle and, in many cases, causes an increase in the specific productivity of recombinant protein. Controlled proliferation is often used to increase CHO specific productivity, and thus there is speculation that the enhanced productivity at low temperature is due to G0/G1-phase growth arrest. However, we show that the positive effect of low temperature on recombinant protein production is due to elevated mRNA levels and not due to growth arrest and that a cell line can still exhibit growth-associated productivity at low temperatures. Using a CHO cell producing recombinant human IFN-γ (interferon-γ), we show that productivity increases as the percentage of cells in the S phase of the cell cycle increases, at both 32 and 37 °C. The increased productivity is due to higher recombinant IFN-γ mRNA levels. We also show that, for a given cell-cycle distribution, specific productivity increases as the temperature is lowered from 37 to 32 °C. Thus specific productivity is maximized when cells are actively growing (high percentage of S-phase cells) and also exposed to low temperature. These findings have important implications for cell-culture optimization and cell-line engineering, providing evidence that a CHO cell line capable of actively growing at low temperature would provide improved total protein production relative to the current growth strategies, namely 37 °C active growth or low-temperature growth arrest. © 2005 Portland Press Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1042/BA20040066
dc.sourceScopus
dc.subjectControlled proliferation
dc.subjectGrowth arrest
dc.subjectHypothermia
dc.subjectInterferon-γ
dc.subjectLow temperature
dc.subjectReal-time PCR
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentMATERIALS SCIENCE AND ENGINEERING
dc.description.doi10.1042/BA20040066
dc.description.sourcetitleBiotechnology and Applied Biochemistry
dc.description.volume41
dc.description.issue3
dc.description.page255-264
dc.description.codenBABIE
dc.identifier.isiut000229901400009
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