Please use this identifier to cite or link to this item: https://doi.org/10.1039/b401834f
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dc.titleFilter-based microfluidic device as a platform for immunofluorescent assay of microbial cells
dc.contributor.authorZhu, L.
dc.contributor.authorZhang, Q.
dc.contributor.authorFeng, H.
dc.contributor.authorAng, S.
dc.contributor.authorChau, F.S.
dc.contributor.authorLiu, W.-T.
dc.date.accessioned2014-10-07T06:27:00Z
dc.date.available2014-10-07T06:27:00Z
dc.date.issued2004-08
dc.identifier.citationZhu, L., Zhang, Q., Feng, H., Ang, S., Chau, F.S., Liu, W.-T. (2004-08). Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells. Lab on a Chip - Miniaturisation for Chemistry and Biology 4 (4) : 337-341. ScholarBank@NUS Repository. https://doi.org/10.1039/b401834f
dc.identifier.issn14730197
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/84595
dc.description.abstractA filter-based microfluidic device was combined with immunofluorescent labeling as a platform to rapidly detect microbial cells. The coin-sized device consisted of micro-chambers, micro-channels and filter weirs (gap = 1-2 μm), and was demonstrated to effectively trap and concentrate microbial cells (i.e., Cryptosporidium parvum and Giardia lamblia), which were larger in size than the weir gap. After sample injection, a staining solution containing fluorescently-labeled antibodies was continuously provided into the device (flow rate = 20 μl min-1) to flush the microbial cells toward the weirs and to accelerate the fluorescent labeling reaction. Using a staining solution that was 10 to 100 times more dilute than the recommended concentration used in a conventional glass method, those target cells with a fluorescent signal-to-noise ratio of 12 could be microscopically observed at single-cell level within 2 to 5 min prior to secondary washing.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1039/b401834f
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCIVIL ENGINEERING
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1039/b401834f
dc.description.sourcetitleLab on a Chip - Miniaturisation for Chemistry and Biology
dc.description.volume4
dc.description.issue4
dc.description.page337-341
dc.description.codenLCAHA
dc.identifier.isiut000222814200013
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