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Title: Characterization of microbial community in granular sludge treating brewery wastewater
Authors: Liu, W.-T. 
Chan, O.-C.
Fang, H.H.P.
Keywords: 16S rRNA
Anaerobic biogranule
Fluorescence in-situ hybridization
Microbial community
Oligonucleotide probes
Issue Date: 2002
Citation: Liu, W.-T., Chan, O.-C., Fang, H.H.P. (2002). Characterization of microbial community in granular sludge treating brewery wastewater. Water Research 36 (7) : 1767-1775. ScholarBank@NUS Repository.
Abstract: The diversity and distribution of microbes within brewery-degrading anaerobic sludge granules were studied using various molecular techniques. Molecular cloning of small-subunit rRNA gene sequences indicated that all archaeal clones were affiliated with Methanosaeta concillii (>99% sequence similarity), and the bacterial clones were mostly affiliated with a not-yet-cultured Clostridium cluster (48 out of 99 clones) in the low G+C gram-positive group, Xanthomonas spp. in the γ-subclass of Proteobacteria (30 clones), and Desulfovibrio spp. (16 clones) in the δ-subclass of Proteobacteria. Slot-blot hybridization indicated that archaeal cells from the Methanomicrobiales (58.4% of total rRNA), Methanobacterials (3.3%) and Methanococcales (1.0%) accounted for 62.4% of the total community rRNA. The rest of the microbial populations were the clostridial cluster (27.3% of total rRNA) and Desulfovibrio spp. (9.4%). Fluorescence in-situ hybridization with domain and group-specific oligonucleotide probes further revealed a multi-layer granular architecture. On the surface layer, the hydrolytic clostridial species and hydrogenotrophic Methanobacteriales were the predominant. In the middle layer, mostly H2-producing acetogens from the δ-Proteobacteria (i.e., Desulfovibrio spp.), hydrogenotrophic Methanobacteriales and aceticlastic Methanosaeta were observed to presumably form a syntrophic association. Finally, the center core consisted of microcolonies of Methanosaeta cells. Copyright © 2002 Elsevier Science Ltd.
Source Title: Water Research
ISSN: 00431354
DOI: 10.1016/S0043-1354(01)00377-3
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