Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.ab.2011.05.045
| DC Field | Value | |
|---|---|---|
| dc.title | Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay | |
| dc.contributor.author | Vashist, S.K. | |
| dc.contributor.author | Zhang, B. | |
| dc.contributor.author | Zheng, D. | |
| dc.contributor.author | Al-Rubeaan, K. | |
| dc.contributor.author | Luong, J.H.T. | |
| dc.contributor.author | Sheu, F.-S. | |
| dc.date.accessioned | 2014-10-07T04:37:25Z | |
| dc.date.available | 2014-10-07T04:37:25Z | |
| dc.date.issued | 2011-10 | |
| dc.identifier.citation | Vashist, S.K., Zhang, B., Zheng, D., Al-Rubeaan, K., Luong, J.H.T., Sheu, F.-S. (2011-10). Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay. Analytical Biochemistry 417 (1) : 156-158. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ab.2011.05.045 | |
| dc.identifier.issn | 00032697 | |
| dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/83113 | |
| dc.description.abstract | This study revealed a major interference from sulfo-N-hydroxysuccinimide (sulfo-NHS) in the bicinchoninic acid (BCA) protein assay. Sulfo-NHS, a common reagent used in bioconjugation and analytical biochemistry, exhibited absorbance signals and absorbance peaks at 562 nm, comparable to bovine serum albumin (BSA). However, the combined absorbance of sulfo-NHS and BSA was not strictly additive. The sulfo-NHS interference was suggested to be caused by the reduction of Cu 2+ in the BCA Kit's reagent B (4% cupric sulfate) in a manner similar to that of the protein. Crown Copyright © 2011 Published by Elsevier Inc. All rights reserved. | |
| dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ab.2011.05.045 | |
| dc.source | Scopus | |
| dc.type | Article | |
| dc.contributor.department | ELECTRICAL & COMPUTER ENGINEERING | |
| dc.description.doi | 10.1016/j.ab.2011.05.045 | |
| dc.description.sourcetitle | Analytical Biochemistry | |
| dc.description.volume | 417 | |
| dc.description.issue | 1 | |
| dc.description.page | 156-158 | |
| dc.description.coden | ANBCA | |
| dc.identifier.isiut | 000293548400020 | |
| Appears in Collections: | Staff Publications | |
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