Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ab.2011.05.045
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dc.titleSulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay
dc.contributor.authorVashist, S.K.
dc.contributor.authorZhang, B.
dc.contributor.authorZheng, D.
dc.contributor.authorAl-Rubeaan, K.
dc.contributor.authorLuong, J.H.T.
dc.contributor.authorSheu, F.-S.
dc.date.accessioned2014-10-07T04:37:25Z
dc.date.available2014-10-07T04:37:25Z
dc.date.issued2011-10
dc.identifier.citationVashist, S.K., Zhang, B., Zheng, D., Al-Rubeaan, K., Luong, J.H.T., Sheu, F.-S. (2011-10). Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay. Analytical Biochemistry 417 (1) : 156-158. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ab.2011.05.045
dc.identifier.issn00032697
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/83113
dc.description.abstractThis study revealed a major interference from sulfo-N-hydroxysuccinimide (sulfo-NHS) in the bicinchoninic acid (BCA) protein assay. Sulfo-NHS, a common reagent used in bioconjugation and analytical biochemistry, exhibited absorbance signals and absorbance peaks at 562 nm, comparable to bovine serum albumin (BSA). However, the combined absorbance of sulfo-NHS and BSA was not strictly additive. The sulfo-NHS interference was suggested to be caused by the reduction of Cu 2+ in the BCA Kit's reagent B (4% cupric sulfate) in a manner similar to that of the protein. Crown Copyright © 2011 Published by Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ab.2011.05.045
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentELECTRICAL & COMPUTER ENGINEERING
dc.description.doi10.1016/j.ab.2011.05.045
dc.description.sourcetitleAnalytical Biochemistry
dc.description.volume417
dc.description.issue1
dc.description.page156-158
dc.description.codenANBCA
dc.identifier.isiut000293548400020
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