Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.memsci.2012.03.004
DC FieldValue
dc.titlePlanar biomimetic aquaporin-incorporated triblock copolymer membranes on porous alumina supports for nanofiltration
dc.contributor.authorDuong, P.H.H.
dc.contributor.authorChung, T.-S.
dc.contributor.authorJeyaseelan, K.
dc.contributor.authorArmugam, A.
dc.contributor.authorChen, Z.
dc.contributor.authorYang, J.
dc.contributor.authorHong, M.
dc.date.accessioned2014-10-07T04:35:01Z
dc.date.available2014-10-07T04:35:01Z
dc.date.issued2012-08-01
dc.identifier.citationDuong, P.H.H., Chung, T.-S., Jeyaseelan, K., Armugam, A., Chen, Z., Yang, J., Hong, M. (2012-08-01). Planar biomimetic aquaporin-incorporated triblock copolymer membranes on porous alumina supports for nanofiltration. Journal of Membrane Science 409-410 : 34-43. ScholarBank@NUS Repository. https://doi.org/10.1016/j.memsci.2012.03.004
dc.identifier.issn03767388
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/82914
dc.description.abstractThe aim of the present study was to investigate and demonstrate the preparation and characterization of planar biomimetic water-channel protein Aquaporin Z (AqpZ)-incorporated polymer membranes on different porous alumina substrates by vesicle spreading. AqpZ-incorporated polymer vesicles were prepared from disulfide-functionalized poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-poly(2-methyloxazoline) (disulfide-functionalized PMOXA 20-PDMS 75-PMOXA 20) tri-block copolymer which was previously proven to be able to maintain protein activity after the incorporation as found in lipid vesicles. AqpZ-incorporated vesicle permeability was evaluated using stopped-flow spectroscopy. The permeability of AqpZ-vesicles measured by stopped-flow spectroscopy was 4680μm/s at room temperature when the molar ratio of AqpZ/polymer used to prepare the vesicles was 1/50. The result demonstrates that AqpZ could maintain its performance after inserting into polymer vesicles. In addition, the vesicles were designed with disulfide functional end groups to enhance vesicle spreading on gold-coated solid substrates by covalent interactions between polymer membranes and substrates. The morphology of polymer membranes on different types of porous alumina substrates was elucidated by using atomic force microscopy (AFM) and field-emission scanning electron microscopy (FESEM). AqpZ-incorporated polymer vesicles were demonstrated to be able to form planar AqpZ-incorporated polymer membranes on the porous alumina substrates. Preliminary studies of membrane's permeability showed that AqpZ were still in the active form on the planar membranes and the resultant membranes may be useful tools for nanofiltration process for water reuse. © 2012 Elsevier B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.memsci.2012.03.004
dc.sourceScopus
dc.subjectAquaporin Z
dc.subjectBiomimetic membrane
dc.subjectNanofiltration
dc.subjectPolymer vesicles
dc.typeArticle
dc.contributor.departmentELECTRICAL & COMPUTER ENGINEERING
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.memsci.2012.03.004
dc.description.sourcetitleJournal of Membrane Science
dc.description.volume409-410
dc.description.page34-43
dc.description.codenJMESD
dc.identifier.isiut000303775400005
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

56
checked on Sep 13, 2019

WEB OF SCIENCETM
Citations

55
checked on Sep 5, 2019

Page view(s)

51
checked on Sep 7, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.