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https://scholarbank.nus.edu.sg/handle/10635/80215
Title: | CAN RANDOM PROCESSES CREATE ROBUST BEHAVIOUR IN CLATHRIN-MEDIATED ENDOCYTOSIS? AN IN-SILICO STUDY OF THE SELFASSEMBLY OF CLATHRIN AND PUTATIVE ROLES FOR THE DISORDERED REGIONS OF EPSIN AND EPS15. | Authors: | NARENDRA SUHAS JAGANNATHAN | Keywords: | Stochasticity, Self-assembly, Clathrin, Modelling, IDP, Endocytosis | Issue Date: | 31-Mar-2014 | Citation: | NARENDRA SUHAS JAGANNATHAN (2014-03-31). CAN RANDOM PROCESSES CREATE ROBUST BEHAVIOUR IN CLATHRIN-MEDIATED ENDOCYTOSIS? AN IN-SILICO STUDY OF THE SELFASSEMBLY OF CLATHRIN AND PUTATIVE ROLES FOR THE DISORDERED REGIONS OF EPSIN AND EPS15.. ScholarBank@NUS Repository. | Abstract: | Stochasticity is an intrinsic feature of many natural processes. In this thesis, we study two processes in Clathrin-Mediated Endocytosis to see if stochastic processes at one scale might result in deterministic outcomes at a higher scale. The two processes are - the self-assembly of the coat protein Clathrin into cages, and the binding of the protein AP2 to long disordered regions in proteins Epsin and Eps15. To study Clathrin self-assembly, we design a Monte-Carlo simulating engine - CLASS (CLathrin ASsembly Simulator). We use this simulator to study how geometry and configuration-dependent binding affinities between Clathrin molecules might affect the morphologies of the lattices they form. To study Epsin/Eps15 we generate ensembles of structures of the disordered regions and study how the dimensions and energies of the structures vary when bound to multiple copies of a binding partner (AP2a). We speculate that the disorder of these regions, coupled to multiple AP2 binding sites, might be the basis for a deterministic role for these proteins in CME. | URI: | http://scholarbank.nus.edu.sg/handle/10635/80215 |
Appears in Collections: | Ph.D Theses (Open) |
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Clathrin_SelfAssembly_movie.avi | 1.78 MB | AVI | OPEN | None | Preview online | |
Jagannathan_NarendraSuhas.pdf | 8.05 MB | Adobe PDF | OPEN | None | View/Download |
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