Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/80215
Title: CAN RANDOM PROCESSES CREATE ROBUST BEHAVIOUR IN CLATHRIN-MEDIATED ENDOCYTOSIS? AN IN-SILICO STUDY OF THE SELFASSEMBLY OF CLATHRIN AND PUTATIVE ROLES FOR THE DISORDERED REGIONS OF EPSIN AND EPS15.
Authors: NARENDRA SUHAS JAGANNATHAN
Keywords: Stochasticity, Self-assembly, Clathrin, Modelling, IDP, Endocytosis
Issue Date: 31-Mar-2014
Citation: NARENDRA SUHAS JAGANNATHAN (2014-03-31). CAN RANDOM PROCESSES CREATE ROBUST BEHAVIOUR IN CLATHRIN-MEDIATED ENDOCYTOSIS? AN IN-SILICO STUDY OF THE SELFASSEMBLY OF CLATHRIN AND PUTATIVE ROLES FOR THE DISORDERED REGIONS OF EPSIN AND EPS15.. ScholarBank@NUS Repository.
Abstract: Stochasticity is an intrinsic feature of many natural processes. In this thesis, we study two processes in Clathrin-Mediated Endocytosis to see if stochastic processes at one scale might result in deterministic outcomes at a higher scale. The two processes are - the self-assembly of the coat protein Clathrin into cages, and the binding of the protein AP2 to long disordered regions in proteins Epsin and Eps15. To study Clathrin self-assembly, we design a Monte-Carlo simulating engine - CLASS (CLathrin ASsembly Simulator). We use this simulator to study how geometry and configuration-dependent binding affinities between Clathrin molecules might affect the morphologies of the lattices they form. To study Epsin/Eps15 we generate ensembles of structures of the disordered regions and study how the dimensions and energies of the structures vary when bound to multiple copies of a binding partner (AP2a). We speculate that the disorder of these regions, coupled to multiple AP2 binding sites, might be the basis for a deterministic role for these proteins in CME.
URI: http://scholarbank.nus.edu.sg/handle/10635/80215
Appears in Collections:Ph.D Theses (Open)

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