Please use this identifier to cite or link to this item: https://doi.org/10.1038/jid.2012.384
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dc.titleDifferentiation of human embryonic stem cells into clinically amenable keratinocytes in an autogenic environment
dc.contributor.authorKidwai, F.K.
dc.contributor.authorLiu, H.
dc.contributor.authorToh, W.S.
dc.contributor.authorFu, X.
dc.contributor.authorJokhun, D.S.
dc.contributor.authorMovahednia, M.M.
dc.contributor.authorLi, M.
dc.contributor.authorZou, Y.
dc.contributor.authorSquier, C.A.
dc.contributor.authorPhan, T.T.
dc.contributor.authorCao, T.
dc.date.accessioned2014-09-18T09:22:24Z
dc.date.available2014-09-18T09:22:24Z
dc.date.issued2013-03
dc.identifier.citationKidwai, F.K., Liu, H., Toh, W.S., Fu, X., Jokhun, D.S., Movahednia, M.M., Li, M., Zou, Y., Squier, C.A., Phan, T.T., Cao, T. (2013-03). Differentiation of human embryonic stem cells into clinically amenable keratinocytes in an autogenic environment. Journal of Investigative Dermatology 133 (3) : 618-628. ScholarBank@NUS Repository. https://doi.org/10.1038/jid.2012.384
dc.identifier.issn0022202X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/79887
dc.description.abstractHuman embryonic stem cells (hESCs)-derived keratinocytes hold great clinical and research potential. However, the current techniques are hampered by the use of xenogenic components that limits their clinical application. Here we demonstrated an efficient differentiation of H9 hESCs (H9-hESCs) into keratinocytes (H9-Kert) with the minimum use of animal-derived materials. For differentiation, we established two microenvironment systems originated from H9-hESCs (autogenic microenvironment). These autogenic microenvironment systems consist of an autogenic coculture system (ACC) and an autogenic feeder-free system (AFF). In addition, we showed a stage-specific effect of Activin in promoting keratinocyte differentiation from H9-hESCs while repressing the expression of early neural markers in the ACC system. Furthermore, we also explained the effect of Activin in construction of the AFF system made up of extracellular matrix similar to basement membrane extracted from H9-hESC-derived fibroblasts. H9-Kert differentiated in both systems expressed keratinocyte markers at mRNA and protein levels. H9-Kert were also able to undergo terminal differentiation in high Ca2+ medium. These findings support the transition toward the establishment of an animal-free microenvironment for successful differentiation of hESCs into keratinocytes for potential clinical application. © 2013 The Society for Investigative Dermatology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/jid.2012.384
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDENTISTRY
dc.contributor.departmentORAL AND MAXILLOFACIAL SURGERY
dc.description.doi10.1038/jid.2012.384
dc.description.sourcetitleJournal of Investigative Dermatology
dc.description.volume133
dc.description.issue3
dc.description.page618-628
dc.description.codenJIDEA
dc.identifier.isiut000315008500008
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