Separation of myoglobin molecular mass markers using non-gel sieving capillary electrophoresis

Abstract

A non-gel sieving method was described for the separation of myoglobin molecular mass markers (M(r) range 2512-16 949). In this system glycerol was added in order to slow the diffusion of peptides. The results are consistent with those obtained with cross-linked polyacrylamide. Deviation from linearity in the log M, versus migration time plot was observed for the smaller peptides because of their intrinsic net charge. Compared with traditional slab gel electrophoresis this method is simpler and less time consuming.