Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0039-9140(97)00263-4
DC FieldValue
dc.titlePurification of metallothionein proteins from the crab, Portunus pelagicus - Selectivity of hydrophobic interaction chromatography
dc.contributor.authorAng, S.G.
dc.contributor.authorChong, P.S.
dc.date.accessioned2014-06-23T05:55:37Z
dc.date.available2014-06-23T05:55:37Z
dc.date.issued1998-02
dc.identifier.citationAng, S.G., Chong, P.S. (1998-02). Purification of metallothionein proteins from the crab, Portunus pelagicus - Selectivity of hydrophobic interaction chromatography. Talanta 45 (4) : 693-701. ScholarBank@NUS Repository. https://doi.org/10.1016/S0039-9140(97)00263-4
dc.identifier.issn00399140
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/77481
dc.description.abstractBoth hydrophobic interaction chromatography (HIC) and ion-exchange chromatography (IEC) are commonly used for the purification of proteins as their mild elution conditions preclude the dangers of denaturation. Of the two methods, IEC which fractionates proteins on the basis of charge differences, exhibits a higher selectivity. In the case of the metallothionein (MT) proteins, the charge states of the proteins are very susceptible to change due to oxidation of the abundant thiol groups. This complicates fractionation with IEC. Separation using HIC, on the other hand, is based on the exposed hydrophobic groups, which remain relatively intact if the tertiary structure of the proteins is not disrupted. In this work, the successful isolation of two MT isoforms from the tropical crab species, Portunus pelagicus, using HIC serves to demonstrate the high selectivity of this technique, in addition to the fact that it is indifferent to the state of oxidation of the MT proteins during the purification procedure.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0039-9140(97)00263-4
dc.sourceScopus
dc.subjectHydrophobic interaction chromatography
dc.subjectIon-exchange chromatography
dc.subjectIsoforms
dc.subjectMetallothioneins
dc.subjectPortunus pelagicus
dc.typeConference Paper
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1016/S0039-9140(97)00263-4
dc.description.sourcetitleTalanta
dc.description.volume45
dc.description.issue4
dc.description.page693-701
dc.description.codenTLNTA
dc.identifier.isiut000072165000011
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