Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00216-012-5923-9
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dc.titleA combination of metabolomics and metallomics studies of urine and serum from hypercholesterolaemic rats after berberine injection
dc.contributor.authorLiu, F.
dc.contributor.authorGan, P.P.
dc.contributor.authorWu, H.
dc.contributor.authorWoo, W.S.
dc.contributor.authorOng, E.S.
dc.contributor.authorLi, S.F.Y.
dc.date.accessioned2014-06-23T05:54:26Z
dc.date.available2014-06-23T05:54:26Z
dc.date.issued2012-05
dc.identifier.citationLiu, F., Gan, P.P., Wu, H., Woo, W.S., Ong, E.S., Li, S.F.Y. (2012-05). A combination of metabolomics and metallomics studies of urine and serum from hypercholesterolaemic rats after berberine injection. Analytical and Bioanalytical Chemistry 403 (3) : 847-856. ScholarBank@NUS Repository. https://doi.org/10.1007/s00216-012-5923-9
dc.identifier.issn16182642
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/77389
dc.description.abstractBerberine, long used as a remedy in China and India for intestinal infections, has been discovered in recent years in western countries and is now being used to treat ailments ranging from urinary tract infections to diabetes and obesity. In order to study the effect of berberine more deeply, a combined metabolomic and metallomic approach was developed in this study using the hypercholesterolaemic rat model, which involved the use of proton nuclear magnetic resonance for the analysis of rat urine to achieve metabolic fingerprinting and inductively coupled plasma mass spectrometry for the analysis of rat blood serum to achieve metallomic fingerprinting. The results obtained indicated that major metabolic processes like Krebs cycle, cholesterol metabolism and osmoregulation in hypercholesterolaemic rats are perturbed upon berberine injection. In addition, the changes of some elements, such as V, Mn, Na and K, revealed in the metallomic study may contribute to the search of new biomarkers for hypercholesterolaemic disease. We concluded that both the metabolomic and metallomic profiles of berberinetreated hypercholesterolaemic rats were different from those of the control group and that the selected metabolites and elements could probably be applied as potential biomarkers for the understanding of the effect of berberine on biochemical process in the animal model. Such a multi-analytical approach will potentially provide an information-rich platform for the elucidation of effects of xenobiotics and drug efficacy studies. © Springer-Verlag 2011.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s00216-012-5923-9
dc.sourceScopus
dc.subject1H NMR
dc.subjectBerberine
dc.subjectICP-MS
dc.subjectMetabolomics
dc.subjectMetallomics
dc.typeConference Paper
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentNUS ENVIRONMENTAL RESEARCH INSTITUTE
dc.description.doi10.1007/s00216-012-5923-9
dc.description.sourcetitleAnalytical and Bioanalytical Chemistry
dc.description.volume403
dc.description.issue3
dc.description.page847-856
dc.description.codenABCNB
dc.identifier.isiut000303409600024
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