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Title: Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniques
Authors: Liu, J. 
Saw, C.L.L.
Olivo, M. 
Sudhaharan, T.
Ahmed, S.
Heng, P.W.S. 
Wohland, T. 
Keywords: Chick chorioallantoic membrane (CAM)
Digitized fluorescence endoscopic imaging (DFEI)
Drug delivery
Fluorescence correlation spectroscopy (FCS)
Fluorescence lifetime imaging microscopy (FLIM)
Hypericin (HY)
N-methyl pyrrolidone (NMP)
Photodynamic therapy and diagnosis
Polyvinylpyrrolidone (PVP)
Issue Date: 2009
Citation: Liu, J., Saw, C.L.L., Olivo, M., Sudhaharan, T., Ahmed, S., Heng, P.W.S., Wohland, T. (2009). Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniques. Journal of Biomedical Optics 14 (1) : -. ScholarBank@NUS Repository.
Abstract: We report the detection of interactions between a photosensitizer, hypericin (HY), and its solvent system prepared with a formulation additive, polyvinylpyrrolidone (PVP), a commonly used pharmaceutical excipient. Fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging microscopy (FLIM) were used to study aggregation and binding of HY in the presence of PVP. Digitized fluorescence endoscopic imaging (DFEI) was used to study the effect of the pharmaceutical formulation in the in vivo tumor implanted chick chorioallantoic membrane (CAM) model. The results presented reveal the coordination of HY-PVP binding, HY disaggregation in the presence of PVP, and strengthened HY tumor uptake selectivity. PVP is thus suggested as a potential adjuvant to previously investigated N-methyl pyrrolidone (NMP) in the HY delivery system as well as a replacement for the conventionally used albumin in the HY bladder instillation fluids preparation for clinical use. © 2009 Society of Photo-Optical Instrumentation Engineers.
Source Title: Journal of Biomedical Optics
ISSN: 10833668
DOI: 10.1117/1.3067726
Appears in Collections:Staff Publications

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