Please use this identifier to cite or link to this item: https://doi.org/10.1021/jf049112q
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dc.titleScreening and identification of antioxidants in biological samples using high-performance liquid chromatography-mass spectrometry and its application on Salacca edulis Reinw
dc.contributor.authorShui, G.
dc.contributor.authorLeong, L.P.
dc.date.accessioned2014-06-23T05:48:43Z
dc.date.available2014-06-23T05:48:43Z
dc.date.issued2005-02-23
dc.identifier.citationShui, G., Leong, L.P. (2005-02-23). Screening and identification of antioxidants in biological samples using high-performance liquid chromatography-mass spectrometry and its application on Salacca edulis Reinw. Journal of Agricultural and Food Chemistry 53 (4) : 880-886. ScholarBank@NUS Repository. https://doi.org/10.1021/jf049112q
dc.identifier.issn00218561
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/76921
dc.description.abstractIn this study, a new approach was developed for screening and identifying antioxidants in biological samples. The approach was based on significant decreases of the intensities of ion peaks obtained from high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) upon reaction with 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals. HPLC-MS/MS was further applied to elucidate structures of antioxidant peaks characterized in a spiking test. The new approach could also be used to monitor the reactivity of antioxidants in biological sample with free radicals. The approach was successfully applied to the identification of antioxidants in salak (Salacca edulis Reinw), a tropical fruit that is reported to be a very good source of natural antioxidants, but it was still not clear which compounds were responsible for its antioxidant property. The antioxidants in salak were identified to be chlorogenic acid, (-)-epicatechin, and singly linked proanthocyanidins that mainly existed as dimers through hexamers of catechin or epicatechin. In salak, chlorogenic acid was identified to be an antioxidant of the slow reaction type as it reacted with free radicals much more slowly than either (-)-epicatechin or proanthocyanidins. The new approach was proved to be useful for the characterization and identification of antioxidants in biological samples as a mass detector combined with an HPLC separation system not only serves as an ideal tool to monitor free radical active components but also provides their possible chemical structures in a biological sample.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/jf049112q
dc.sourceScopus
dc.subjectABTS free radical
dc.subjectAntioxidant
dc.subjectChlorogenic acid
dc.subjectFlavan-3-ols
dc.subjectHPLC-MS
dc.subjectSalak
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1021/jf049112q
dc.description.sourcetitleJournal of Agricultural and Food Chemistry
dc.description.volume53
dc.description.issue4
dc.description.page880-886
dc.description.codenJAFCA
dc.identifier.isiut000227079800007
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