Please use this identifier to cite or link to this item: https://doi.org/10.1038/nprot.2008.139
DC FieldValue
dc.titlePeptide microarrays for high-throughput studies of Ser/Thr phosphatases
dc.contributor.authorSun, H.
dc.contributor.authorLu, C.H.S.
dc.contributor.authorShi, H.
dc.contributor.authorGao, L.
dc.contributor.authorYao, S.Q.
dc.date.accessioned2014-06-23T05:46:24Z
dc.date.available2014-06-23T05:46:24Z
dc.date.issued2008
dc.identifier.citationSun, H., Lu, C.H.S., Shi, H., Gao, L., Yao, S.Q. (2008). Peptide microarrays for high-throughput studies of Ser/Thr phosphatases. Nature Protocols 3 (9) : 1485-1493. ScholarBank@NUS Repository. https://doi.org/10.1038/nprot.2008.139
dc.identifier.issn17542189
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/76732
dc.description.abstractProtein phosphorylation and dephosphorylation play an important role in regulation of intracellular signal transduction pathways in the biological system. A key step in the biological characterization of phosphatases and their use as drug targets is the identification of their cellular partners and suitable substrates for potential inhibitor development. Herein we describe a microarray-based protocol to map the substrate specificity of protein Ser/Thr phosphatases. This protocol uses Pro-Q dye to sensitively and quantitatively detect the amount of dephosphorylation that occurs from many putative peptide substrates in parallel, and therefore could be used to generate the so-called peptide substrate fingerprints as well as detailed kinetic information of a target phosphatase. Excluding the synthesis of the peptide substrates, the whole protocol takes a total of 11 h to complete and in future can be readily extended to the study of other classes of phosphatases, i.e., protein tyrosine phosphatases.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/nprot.2008.139
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1038/nprot.2008.139
dc.description.sourcetitleNature Protocols
dc.description.volume3
dc.description.issue9
dc.description.page1485-1493
dc.identifier.isiut000266108100006
Appears in Collections:Staff Publications

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