Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2007.05.067
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dc.titleLarge-volume sample stacking with polarity switching for the analysis of bacteria by capillary electrophoresis with laser-induced fluorescence detection
dc.contributor.authorYu, L.
dc.contributor.authorLi, S.F.Y.
dc.date.accessioned2014-06-23T05:42:47Z
dc.date.available2014-06-23T05:42:47Z
dc.date.issued2007-08-17
dc.identifier.citationYu, L., Li, S.F.Y. (2007-08-17). Large-volume sample stacking with polarity switching for the analysis of bacteria by capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography A 1161 (1-2) : 308-313. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2007.05.067
dc.identifier.issn00219673
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/76437
dc.description.abstractCapillary electrophoresis has been utilized for the rapid analysis of bacteria under specific experimental conditions. In this work, a method of large-volume sample stacking with polarity switching was evaluated for the analysis of bacteria by capillary electrophoresis with laser-induced fluorescence detection in order to enhance the detection sensitivity. The results indicated that the proposed method is not only effective for the focusing of bacterial cells, but also for the separation of mixtures of bacteria. With the optimized conditions, an enhancement factor of around 60-fold was obtained when long sample plug (up to 39.6% of capillary volume) was injected. Moreover, with the help of such stacking method, single, sharp, intense peak with high efficiency was observed without multiple peaks attributable to irregular clusters and aggregates of bacterial cells. This simple stacking approach appears to be promising as a rapid sterility test in various fields of applications. © 2007 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.chroma.2007.05.067
dc.sourceScopus
dc.subjectBacteria
dc.subjectCapillary electrophoresis
dc.subjectLarge-volume sample stacking
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1016/j.chroma.2007.05.067
dc.description.sourcetitleJournal of Chromatography A
dc.description.volume1161
dc.description.issue1-2
dc.description.page308-313
dc.description.codenJCRAE
dc.identifier.isiut000248891500039
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