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Title: Immunoassay by capillary electrophoresis with quantum dots
Authors: Feng, H.-t.
Law, W.-S. 
Yu, L.-J. 
Li, S.F.-Y. 
Keywords: Capillary electrophoresis
Laser-induced fluorescence
Quantum dots
Issue Date: 13-Jul-2007
Citation: Feng, H.-t., Law, W.-S., Yu, L.-J., Li, S.F.-Y. (2007-07-13). Immunoassay by capillary electrophoresis with quantum dots. Journal of Chromatography A 1156 (1-2 SPEC. ISS.) : 75-79. ScholarBank@NUS Repository.
Abstract: The application of quantum dots in capillary electrophoresis immunoassay was studied for the first time. Quantum dots were conjugated with antibody and subsequently tested by electrophoretic separation of free antibody and antibody-antigen complex. Antibody was fluorescently labeled by quantum dots via conjugation procedures and its electrophoretic characteristics were effectively modified due to the attachment of quantum dots. The determination of human IgM by direct CE based immunoassay could be easily achieved by simply changing the pH value of separation buffer. Polymer additive influenced the separation too but the effect was not as significant as buffer pH adjustment. Satisfactory separation of complex from free antibody could be achieved with 20 mM sodium tetraborate as separation buffer, at pH 9.8. The immunoassay application of quantum dots in CE offers considerable advantages and can be readily applied to other large bio-molecules. © 2007 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography A
ISSN: 00219673
DOI: 10.1016/j.chroma.2006.12.077
Appears in Collections:Staff Publications

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