Please use this identifier to cite or link to this item:
DC FieldValue
dc.titleGrowing tissue-like constructs with Hep3B/HepG2 liver cells on PHBV microsphere scaffold
dc.contributor.authorZhu, X.H.
dc.contributor.authorWang, C.-H.
dc.contributor.authorTong, Y.W.
dc.identifier.citationZhu, X.H.,Wang, C.-H.,Tong, Y.W. (2006). Growing tissue-like constructs with Hep3B/HepG2 liver cells on PHBV microsphere scaffold. AIChE Annual Meeting, Conference Proceedings : -. ScholarBank@NUS Repository.
dc.description.abstractIn this study, a solvent evaporation technique was used to fabricate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV, 8% PHV) microspheres as scaffold to guide liver cell growth. Human hepatoma cell lines, HepG2 and Hep3B, were cultured in vitro on both the microspheres and polymer films. SEM and optical microscope images showed that multilayer cells were formed among the microspheres to bridge them together and developed into cell-construct aggregates after one week of culture. MTT results showed that the cell proliferation on the microspheres was more than two times higher than that on the films after 12 days of culture. The cells seeded on microspheres secreted albumin 2-4 times more than that on the positive control after one weeks of culture, which indicated that this hepatic function was greatly improved by the aggregation of cells on microspheres. Although HepG2 failed to express P-450 activity, this hepatic function was preserved when Hep3B cultured on microspheres. All the results indicated that PHBV microspheres are appropriate scaffolds for liver tissue engineering.
dc.subjectLiver cells
dc.subjectLiver tissue engineering
dc.typeConference Paper
dc.description.sourcetitleAIChE Annual Meeting, Conference Proceedings
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.