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https://scholarbank.nus.edu.sg/handle/10635/73240
| DC Field | Value | |
|---|---|---|
| dc.title | Cell cycle synchronization of stem cells using inertial microfluidics | |
| dc.contributor.author | Lee, W.C. | |
| dc.contributor.author | Bhagat, A.A.S. | |
| dc.contributor.author | Huang, S. | |
| dc.contributor.author | Van Vliet, K.J. | |
| dc.contributor.author | Han, J. | |
| dc.contributor.author | Lim, C.T. | |
| dc.date.accessioned | 2014-06-19T05:32:47Z | |
| dc.date.available | 2014-06-19T05:32:47Z | |
| dc.date.issued | 2010 | |
| dc.identifier.citation | Lee, W.C.,Bhagat, A.A.S.,Huang, S.,Van Vliet, K.J.,Han, J.,Lim, C.T. (2010). Cell cycle synchronization of stem cells using inertial microfluidics. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 1 : 208-210. ScholarBank@NUS Repository. | |
| dc.identifier.isbn | 9781618390622 | |
| dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/73240 | |
| dc.description.abstract | Cell cycle synchronization is of paramount importance for studying cellular properties and biological processes involved in various stages of the cell cycle. Here, we present a microfluidics based approach to synchronize the cell cycle of a primary - cells, human bone marrow-derived mesenchymal stem cells (hMSCs), using inertial forces in spiral microchan-nels. The device operating principle exploits the relationship between the volume (and thus diameter) of a cell and its phase in the cell cycle, in order to fractionate hMSCs populations into synchronized subpopulations enriched in cell cycle fractions of G0/G1, S and G2/M phases. | |
| dc.source | Scopus | |
| dc.subject | Cell cycle | |
| dc.subject | Dean flows | |
| dc.subject | Inertial forces | |
| dc.subject | Size fractionation | |
| dc.subject | Stem cells | |
| dc.type | Conference Paper | |
| dc.contributor.department | MECHANICAL ENGINEERING | |
| dc.description.sourcetitle | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 | |
| dc.description.volume | 1 | |
| dc.description.page | 208-210 | |
| dc.identifier.isiut | NOT_IN_WOS | |
| Appears in Collections: | Staff Publications | |
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