Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.07.045
DC FieldValue
dc.titleImmobilization of flavin adenine dinucleotide (FAD) onto carbon cloth and its application as working electrode in an electroenzymatic bioreactor
dc.contributor.authorJayabalan, R.
dc.contributor.authorSathishkumar, M.
dc.contributor.authorJeong, E.S.
dc.contributor.authorMun, S.P.
dc.contributor.authorYun, S.E.
dc.date.accessioned2014-06-18T05:09:12Z
dc.date.available2014-06-18T05:09:12Z
dc.date.issued2012-11
dc.identifier.citationJayabalan, R., Sathishkumar, M., Jeong, E.S., Mun, S.P., Yun, S.E. (2012-11). Immobilization of flavin adenine dinucleotide (FAD) onto carbon cloth and its application as working electrode in an electroenzymatic bioreactor. Bioresource Technology 123 : 686-689. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.07.045
dc.identifier.issn09608524
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67770
dc.description.abstractA high porosity carbon cloth with immobilized FAD was employed as working electrode in electrochemical NADH-regeneration procedure. Carbon cloth was oxidized with hot acids to create surface carboxyl group and then coupled by adenine amino group of FAD with carbodiimide in the presence of N-hydroxysulfosuccinimide. The bioelectrocatalytic NADH-regeneration was coupled to the conversion of achiral substrate pyruvate into chiral product l-lactate by l-lactate dehydrogenase (l-LDH) within the same reactor. The conversion was completed at 96 h in bioreactor with FAD-modified carbon cloth, resulting in about 6. mM of l-lactate from 10. mM of pyruvate. While with bare carbon cloth, the yield at 120 h was around 5. mM. Immobilized FAD on the surface of carbon cloth electrode facilitated it to carry electrons from electrode to electron transfer enzymes; thereby NADH-regeneration was accelerated to drive the enzymatic reaction efficiently. © 2012 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biortech.2012.07.045
dc.sourceScopus
dc.subjectBioreduction
dc.subjectCarbon cloth
dc.subjectFlavin adenine dinucleotide
dc.subjectFormate dehydrogenase
dc.subjectNADH regeneration
dc.typeArticle
dc.contributor.departmentSINGAPORE-DELFT WATER ALLIANCE
dc.description.doi10.1016/j.biortech.2012.07.045
dc.description.sourcetitleBioresource Technology
dc.description.volume123
dc.description.page686-689
dc.description.codenBIRTE
dc.identifier.isiut000310401100098
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