Protein micropatterning via self-assembly of nanoparticles

Abstract

A method for patterning proteins on non-planar surfaces via self-assembly of protein-modified silica nanoparticles on microfabricated surfaces was discussed. Silica nanoparticles were prepared by the sol-gel route using tetraethyl orthosilicate (TEOS) as the precursor. Fluorescein isothiocyanate-conjugated bovine albumin was attached to the surfaces of silica nanoparticles, and the nanoparticles were deposited into the microwells. The results show that this technique can be extended to the micropatterning of other biological molecules.