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Title: Investigation of the point spread function of surface plasmon-coupled emission microscopy
Authors: Tang, W.T. 
Chung, E.
Kim, Y.-H.
So, P.T.C.
Sheppard, C.J.R. 
Issue Date: 16-Apr-2007
Citation: Tang, W.T., Chung, E., Kim, Y.-H., So, P.T.C., Sheppard, C.J.R. (2007-04-16). Investigation of the point spread function of surface plasmon-coupled emission microscopy. Optics Express 15 (8) : 4634-4646. ScholarBank@NUS Repository.
Abstract: Surface plasmon-coupled emission microscopy (SPCEM) was proposed as a high sensitivity technique that makes use of a thin layer of metal deposited on glass slides to efficiently excite fluorophores and to collect the emission light. However, the theoretical aspect of SPCEM imaging has not been well studied. We propose a model for SPCEM and show, through theoretical analysis and empirical results, that the point spread function of SPCEM is irregular and has an annular-like structure, significantly different from the familiar point spread function of the conventional wide-field microscopy. This result is due to the highly polarized and anisotropic emission caused by the metal layer. © 2007 Optical Society of America.
Source Title: Optics Express
ISSN: 10944087
DOI: 10.1364/OE.15.004634
Appears in Collections:Staff Publications

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