Please use this identifier to cite or link to this item:
https://doi.org/10.1002/adfm.200800388
DC Field | Value | |
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dc.title | Diffusion controlled and temperature stable microcapsule reaction compartments for high-throughput microcapsule-PCR | |
dc.contributor.author | Mak, W.C. | |
dc.contributor.author | Cheung, K.Y. | |
dc.contributor.author | Trau, D. | |
dc.date.accessioned | 2014-06-17T09:43:18Z | |
dc.date.available | 2014-06-17T09:43:18Z | |
dc.date.issued | 2008-10-09 | |
dc.identifier.citation | Mak, W.C., Cheung, K.Y., Trau, D. (2008-10-09). Diffusion controlled and temperature stable microcapsule reaction compartments for high-throughput microcapsule-PCR. Advanced Functional Materials 18 (19) : 2930-2937. ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.200800388 | |
dc.identifier.issn | 1616301X | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/67005 | |
dc.description.abstract | A novel approach to perform a high number of individual polymerase chain reactions (PCR) in microcapsule reaction compartments, termed "Microcapsule-PCR" was developed. Temperature stable microcapsules with a selective permeable capsule wall were constructed by matrix-assisted layer-by-layer (LbL) Encapsulation technique. During the PCR, small molecular weight building blocks - nucleotides (dNTPs) were supplied externally and diffuse through the permeable capsule wall into the interior, while the resulted high molecular weight PCR products were accumulated within the microcapsule. Microcapsules (∼110.8 μm average diameter) filled with a PCR reaction mixture were constructed by an emulsion technique having a 2% agarose core and a capsule formed by LbL coating with poly(allylanune-hydrochloride) and poly(4-styrene-sulfonate). An encapsulation efficiency of 47% (measured for primer-FITC (22 bases)) and 98% PCR efficiency was achieved. Microcapsules formed by eight layers of polyelectrolyte and subjected to PCR cycling (up to 95°C) demonstrated good temperature stability without any significantly changes in DNA retention yield and microcapsule morphology. A multiplex Microcapsule-PCR experiment demonstrated that microcapsules are individual compartment and do not exchange templates or primers between microcapsules during PCR cycling. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/adfm.200800388 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | BIOENGINEERING | |
dc.description.doi | 10.1002/adfm.200800388 | |
dc.description.sourcetitle | Advanced Functional Materials | |
dc.description.volume | 18 | |
dc.description.issue | 19 | |
dc.description.page | 2930-2937 | |
dc.description.coden | AFMDC | |
dc.identifier.isiut | 000260398300010 | |
Appears in Collections: | Staff Publications |
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