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https://doi.org/10.1023/A:1005613406948
| Title: | Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum | Authors: | Mathew, M. Obbard, J.P. |
Keywords: | Beach sediments Dehydrogenase Hydrocarbon Redox measurements Tetrazolium salts |
Issue Date: | 2001 | Citation: | Mathew, M., Obbard, J.P. (2001). Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum. Biotechnology Letters 23 (3) : 227-230. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1005613406948 | Abstract: | A dehydrogenase enzyme assay has been developed to measure microbial activity in petroleum-contaminated beach sediments. The assay, using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazoliumchloride (INT) as the substrate provides a reliable measurement of overall indigenous microbiological activity in beach sediments containing ∼7% (w/w) oil. The method has been effectively used in field studies (Mathew et al. 1999) to monitor microbial activity in conjunction with the biodegradation of petroleum hydrocarbons. | Source Title: | Biotechnology Letters | URI: | http://scholarbank.nus.edu.sg/handle/10635/66724 | ISSN: | 01415492 | DOI: | 10.1023/A:1005613406948 |
| Appears in Collections: | Staff Publications |
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