Alternative Immunofluorescent labeling of cryptosporidium parvum in water samples using semiconductor quantum dots

Abstract

The application of immunofluorescent labeling using quantum dots for detection of inactivated Cryptosporidium parvum oocysts in spiked water samples (reservoir water, treated wastewater effluent, permeate of a membrane bioreactor, and tap water) provided more consistent results compared with the organic fluorophores label. The varying degree of particles present in the different water samples (with turbidity ranging from 0.2 to 6.1 NTU) in nonconcentrated water samples had insignificant interference on the labeled counts (2-sample t-tests, p < 0.236) using the quantum dot label, while the quantum dot label provided an advantage of approximately 50% lower interference in concentrated water samples compared with the organic fluorophores label.