Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cattod.2009.09.001
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dc.titleIn situ monitoring of turbid immobilized lipase-catalyzed esterification of oleic acid using fiber-optic Raman spectroscopy
dc.contributor.authorElfanso, E.
dc.contributor.authorGarland, M.
dc.contributor.authorLoh, K.C.
dc.contributor.authorTalukder, M.M.R.
dc.contributor.authorWidjaja, E.
dc.date.accessioned2014-06-17T07:43:02Z
dc.date.available2014-06-17T07:43:02Z
dc.date.issued2010-10-15
dc.identifier.citationElfanso, E., Garland, M., Loh, K.C., Talukder, M.M.R., Widjaja, E. (2010-10-15). In situ monitoring of turbid immobilized lipase-catalyzed esterification of oleic acid using fiber-optic Raman spectroscopy. Catalysis Today 155 (3-4) : 223-226. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cattod.2009.09.001
dc.identifier.issn09205861
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/64082
dc.description.abstractRaman spectroscopy with a fiber-optic probe was used to monitor the immobilized Candida antarctica lipase B (Novozym 435)-catalyzed esterification of oleic acid with ethanol in iso-octane as an organic solvent. The effects of reaction temperature and molar ratio of substrates were studied. An optimal reaction rate was found at 60 °C, beyond which deactivation due to denaturing of the lipase was observed. The variation in molar ratio of substrates suggests that the esterification of oleic acid and ethanol proceeds via a Ping-Pong Bi-Bi mechanism with ethanol exhibiting an inhibitory effect. A good fit was obtained between the experimental results and the best-fit Ping-Pong Bi-Bi mechanism. This current work shows that fiber-optic Raman spectroscopy is indeed a suitable instrument to monitor immobilized lipase-catalyzed reaction in turbid organic systems in situ. Since this approach is reliable, simple to use, allows automatic data acquisition, and accurate, it should be applicable to the detailed kinetic analysis on other immobilized enzymatic reactions as well. © 2009 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.cattod.2009.09.001
dc.sourceScopus
dc.subjectEsterification
dc.subjectFiber-optic Raman spectroscopy
dc.subjectIn situ monitoring
dc.subjectLipase
dc.subjectNovozym 435
dc.subjectTurbid systems
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.cattod.2009.09.001
dc.description.sourcetitleCatalysis Today
dc.description.volume155
dc.description.issue3-4
dc.description.page223-226
dc.description.codenCATTE
dc.identifier.isiut000282111200011
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