Please use this identifier to cite or link to this item: https://doi.org/10.4014/jmb.0700.705
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dc.titleExploring the effects of carbon sources on the metabolic capacity for shikimic acid production in Escherichia coli using in silico metabolic predictions
dc.contributor.authorAhn, J.O.
dc.contributor.authorLee, H.W.
dc.contributor.authorSaha, R.
dc.contributor.authorPark, M.S.
dc.contributor.authorJung, J.-K.
dc.contributor.authorLee, D.-Y.
dc.date.accessioned2014-06-17T07:40:50Z
dc.date.available2014-06-17T07:40:50Z
dc.date.issued2008-11-28
dc.identifier.citationAhn, J.O., Lee, H.W., Saha, R., Park, M.S., Jung, J.-K., Lee, D.-Y. (2008-11-28). Exploring the effects of carbon sources on the metabolic capacity for shikimic acid production in Escherichia coli using in silico metabolic predictions. Journal of Microbiology and Biotechnology 18 (11) : 1773-1784. ScholarBank@NUS Repository. https://doi.org/10.4014/jmb.0700.705
dc.identifier.issn10177825
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63896
dc.description.abstractEffects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-scale in silico model of E. coli was conducted to quantify the theoretical maximum SA yield. The corresponding flux distributions fueled by different carbon sources under investigation were compared with respect to theoretical yield and energy utilization, thereby identifying the indispensable pathways for achieving optimal SA production on each carbon source. Subsequently, a shikimate-kinase-deficient E. coli mutant was developed by blocking the aromatic amino acid pathway, and the production of SA on various carbon sources was experimentally examined during 51 batch culture. As a result, the highest production rate, 1.92 mmol SA/h, was obtained when glucose was utilized as a carbon source, whereas the efficient SA production from glycerol was obtained with the highest yield, 0.21 mol SA formed per mol carbon atom of carbon source consumed. The current strain can be further improved to satisfy the theoretically achievable SA production that was predicted by in silico analysis. © The Korean Society for Microbiology and Biotechnology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.4014/jmb.0700.705
dc.sourceScopus
dc.subjectCarbon sources
dc.subjectConstraints-based flux analysis
dc.subjectEscherichia coli
dc.subjectGenome-scale in silico model
dc.subjectShikimic acid production
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.4014/jmb.0700.705
dc.description.sourcetitleJournal of Microbiology and Biotechnology
dc.description.volume18
dc.description.issue11
dc.description.page1773-1784
dc.description.codenJOMBE
dc.identifier.isiut000261237000006
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