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https://doi.org/10.1007/s10616-004-6173-2
DC Field | Value | |
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dc.title | Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures | |
dc.contributor.author | Wong, V.V.T. | |
dc.contributor.author | Ho, K.W. | |
dc.contributor.author | Yap, M.G.S. | |
dc.date.accessioned | 2014-06-17T07:40:34Z | |
dc.date.available | 2014-06-17T07:40:34Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Wong, V.V.T., Ho, K.W., Yap, M.G.S. (2004). Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures. Cytotechnology 45 (3) : 107-115. ScholarBank@NUS Repository. https://doi.org/10.1007/s10616-004-6173-2 | |
dc.identifier.issn | 09209069 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/63874 | |
dc.description.abstract | Insulin is involved in a number of cellular functions, including the stimulation of cell growth, cell cycle progression and glucose uptake and is a common protein supplement in serum-free mammalian cell culture media. However, several trace metals have previously been reported to exhibit insulin-like effects on specific cell types. As a step towards developing chemically-defined, protein-free media for mammalian cells, we tested the effectiveness of five trace metals (cadmium, nickel, lithium, vanadium and zinc) as a replacement for insulin. Four cell lines of biotechnological relevance were used, including the hybridoma CRL1606, the myeloma NS0, and the Chinese hamster ovary cell lines CHO-IFN and CHO-K1. Zinc was found to be an effective insulin replacement for the hybridoma, myeloma and CHO-K1 cells. Cell growth, cell cycle progression and antibody production was not affected by the substitution. Furthermore, no adaptation procedure was required. © 2004 Kluwer Academic Publishers. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s10616-004-6173-2 | |
dc.source | Scopus | |
dc.subject | Insulin | |
dc.subject | Mammalian cell culture | |
dc.subject | Protein-free medium | |
dc.subject | Trace metals | |
dc.subject | Zinc | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1007/s10616-004-6173-2 | |
dc.description.sourcetitle | Cytotechnology | |
dc.description.volume | 45 | |
dc.description.issue | 3 | |
dc.description.page | 107-115 | |
dc.description.coden | CYTOE | |
dc.identifier.isiut | 000228178400002 | |
Appears in Collections: | Staff Publications |
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