Please use this identifier to cite or link to this item: https://doi.org/10.1104/pp.113.220178
DC FieldValue
dc.titleElucidating rice cell metabolism under flooding and drought stresses using flux-based modeling and analysis
dc.contributor.authorLakshmanan, M.
dc.contributor.authorZhang, Z.
dc.contributor.authorMohanty, B.
dc.contributor.authorKwon, J.-Y.
dc.contributor.authorChoi, H.-Y.
dc.contributor.authorNam, H.-J.
dc.contributor.authorKim, D.-I.
dc.contributor.authorLee, D.-Y.
dc.date.accessioned2014-06-17T07:39:56Z
dc.date.available2014-06-17T07:39:56Z
dc.date.issued2013-08
dc.identifier.citationLakshmanan, M., Zhang, Z., Mohanty, B., Kwon, J.-Y., Choi, H.-Y., Nam, H.-J., Kim, D.-I., Lee, D.-Y. (2013-08). Elucidating rice cell metabolism under flooding and drought stresses using flux-based modeling and analysis. Plant Physiology 162 (4) : 2140-2150. ScholarBank@NUS Repository. https://doi.org/10.1104/pp.113.220178
dc.identifier.issn00320889
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63822
dc.description.abstractRice (Oryza sativa) is one of the major food crops in world agriculture, especially in Asia. However, the possibility of subsequent occurrence of flood and drought is a major constraint to its production. Thus, the unique behavior of rice toward flooding and drought stresses has required special attention to understand its metabolic adaptations. However, despite several decades of research investigations, the cellular metabolism of rice remains largely unclear. In this study, in order to elucidate the physiological characteristics in response to such abiotic stresses, we reconstructed what is to our knowledge the first metabolic/regulatory network model of rice, representing two tissue types: germinating seeds and photorespiring leaves. The phenotypic behavior and metabolic states simulated by the model are highly consistent with our suspension culture experiments as well as previous reports. The in silico simulation results of seed-derived rice cells indicated (1) the characteristic metabolic utilization of glycolysis and ethanolic fermentation based on oxygen availability and (2) the efficient sucrose breakdown through sucrose synthase instead of invertase. Similarly, flux analysis on photorespiring leaf cells elucidated the crucial role of plastid-cytosol and mitochondrion-cytosol malate transporters in recycling the ammonia liberated during photorespiration and in exporting the excess redox cofactors, respectively. The model simulations also unraveled the essential role of mitochondrial respiration during drought stress. In the future, the combination of experimental and in silico analyses can serve as a promising approach to understand the complex metabolism of rice and potentially help in identifying engineering targets for improving its productivity as well as enabling stress tolerance. © 2013 American Society of Plant Biologists. All Rights Reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1104/pp.113.220178
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1104/pp.113.220178
dc.description.sourcetitlePlant Physiology
dc.description.volume162
dc.description.issue4
dc.description.page2140-2150
dc.description.codenPLPHA
dc.identifier.isiut000322633400031
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

50
checked on Feb 24, 2021

WEB OF SCIENCETM
Citations

43
checked on Feb 24, 2021

Page view(s)

67
checked on Mar 1, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.