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Title: Carboxylate microsphere-induced cellular toxicity in human lung fibroblasts
Authors: Samy, R.P. 
Ng, C.-T. 
Bay, B.-H.
Watt, F. 
Keywords: Carboxylate microspheres
Lung fibroblasts
Oxidative stress
Issue Date: Jun-2012
Citation: Samy, R.P., Ng, C.-T., Bay, B.-H., Watt, F. (2012-06). Carboxylate microsphere-induced cellular toxicity in human lung fibroblasts. Experimental Biology and Medicine 237 (6) : 635-643. ScholarBank@NUS Repository.
Abstract: Carboxylate microspheres (CMs) are mainly used in industrial, biomedical and various household products. In this study, we assessed the cytotoxic effects of CMs on human MRC-5 lung fibroblasts by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Oxidative stress was determined by measurements of reactive oxygen species and antioxidant (superoxide dismutase and catalase) levels and proinflammatory cytokines quantified by enzyme-linked immunosorbent assay. Morphological changes were examined by light microscopy, confocal microscopy and transmission electron microscopy. The lung fibroblasts were exposed to increasing concentrations of CMs (0.1-1000 μmol/L) for 24 h. The results showed significant changes in cell morphology with induction of cytotoxicity and oxidative stress observed in 10-1000 μmol/L concentrations of CM-treated fibroblasts. Ultrastructural examination revealed the presence of CMs inside the cytoplasm of treated lung fibroblasts. CMs also induced elevated interleukin (IL)-1, IL-6, IL-8, IL-10 and tumor necrosis factor α levels at higher concentrations. We have demonstrated that CMs significantly reduce cell viability in a dose-dependant manner in lung fibroblasts at 0.1-1000 μmol/L doses. The findings suggest that high doses of CMs have the potential to induce cellular toxicity to the lung in vitro. © 2012 by the Society for Experimental Biology and Medicine.
Source Title: Experimental Biology and Medicine
ISSN: 15353702
DOI: 10.1258/ebm.2012.011310
Appears in Collections:Staff Publications

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