Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.08.006
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dc.titleAn in vitro assessment of titanium functionalized with polysaccharides conjugated with vascular endothelial growth factor for enhanced osseointegration and inhibition of bacterial adhesion
dc.contributor.authorHu, X.
dc.contributor.authorNeoh, K.-G.
dc.contributor.authorShi, Z.
dc.contributor.authorKang, E.-T.
dc.contributor.authorPoh, C.
dc.contributor.authorWang, W.
dc.date.accessioned2014-06-17T07:35:49Z
dc.date.available2014-06-17T07:35:49Z
dc.date.issued2010-12
dc.identifier.citationHu, X., Neoh, K.-G., Shi, Z., Kang, E.-T., Poh, C., Wang, W. (2010-12). An in vitro assessment of titanium functionalized with polysaccharides conjugated with vascular endothelial growth factor for enhanced osseointegration and inhibition of bacterial adhesion. Biomaterials 31 (34) : 8854-8863. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.08.006
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63467
dc.description.abstractThe long-term success of orthopedic implants may be compromised by defective osseointegration and bacterial infection. An effective approach to minimize implant failure would be to modify the surface of the implant to make it habitable for bone-forming cells and anti-infective at the same time. In this in vitro study, the surfaces of titanium (Ti) substrates were functionalized by first covalently grafting either dopamine followed by carboxymethyl chitosan (CMCS) or hyaluronic acid-catechol (HAC). Vascular endothelial growth factor (VEGF) was then conjugated to the polysaccharide-grafted surface. Antibacterial assay with Staphylococcus aureus (S. aureus) showed that the polysaccharide-modified substrates significantly decrease bacterial adhesion. The CMCS-functionalized Ti demonstrated better antibacterial property than the HAC-functionalized Ti since CMCS is bactericidal while HA only inhibits the adhesion of bacteria without killing them. Osteoblast attachment, as well as alkaline phosphatase (ALP) activity and calcium deposition were enhanced by the immobilized VEGF on the polysaccharide-grafted Ti. Thus, Ti substrates modified with polysaccharides conjugated with VEGF can promote osteoblast functions and concurrently reduce bacterial adhesion. Since VEGF is also known to enhance angiogenesis, the VEGF-polysaccharide functionalized substrates will have promising applications in the orthopedic field. © 2010 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2010.08.006
dc.sourceScopus
dc.subjectBacterial adhesion
dc.subjectOsteoblasts
dc.subjectPolysaccharide
dc.subjectSurface grafting
dc.subjectVEGF
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.biomaterials.2010.08.006
dc.description.sourcetitleBiomaterials
dc.description.volume31
dc.description.issue34
dc.description.page8854-8863
dc.description.codenBIMAD
dc.identifier.isiut000283814600004
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