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Title: The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage
Authors: Nguyen, L.T.H.
Liao, S.
Ramakrishna, S. 
Chan, C.K. 
Keywords: bone
mesenchymal stem cells
osteogenic differentiation
serial passage
Issue Date: Aug-2011
Citation: Nguyen, L.T.H., Liao, S., Ramakrishna, S., Chan, C.K. (2011-08). The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage. Nanomedicine 6 (6) : 961-974. ScholarBank@NUS Repository.
Abstract: Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold. Materials & Methods: Investigating the influence of serial passages (from passage two to passage eight) on the abilities of proliferation and osteogenic differentiation of hMSCs on 24-well tissue culture polystyrene plates and poly L-lactic acid electrospun nanofibrous scaffolds was performed to determine how prolonged culture affected these cellular abilities and how the nanofibrous scaffolds supported the osteogenic differentiation potential of hMSCs. Results & Conclusion: Serial passage caused adverse changes in hMSCs characteristics, which were indicated by the decline in both proliferation and osteogenic differentiation abilities. Interestingly, the poly L-lactic acid nanofibrous scaffolds showed a significant support in recovering the osteogenic abilities of hMSCs, which had been severely affected by prolonged culture. © 2011 Future Medicine Ltd.
Source Title: Nanomedicine
ISSN: 17435889
DOI: 10.2217/nnm.11.26
Appears in Collections:Staff Publications

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