Please use this identifier to cite or link to this item:
Title: The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage
Authors: Nguyen, L.T.H.
Liao, S.
Ramakrishna, S. 
Chan, C.K. 
Keywords: bone
mesenchymal stem cells
osteogenic differentiation
serial passage
Issue Date: Aug-2011
Citation: Nguyen, L.T.H., Liao, S., Ramakrishna, S., Chan, C.K. (2011-08). The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage. Nanomedicine 6 (6) : 961-974. ScholarBank@NUS Repository.
Abstract: Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold. Materials & Methods: Investigating the influence of serial passages (from passage two to passage eight) on the abilities of proliferation and osteogenic differentiation of hMSCs on 24-well tissue culture polystyrene plates and poly L-lactic acid electrospun nanofibrous scaffolds was performed to determine how prolonged culture affected these cellular abilities and how the nanofibrous scaffolds supported the osteogenic differentiation potential of hMSCs. Results & Conclusion: Serial passage caused adverse changes in hMSCs characteristics, which were indicated by the decline in both proliferation and osteogenic differentiation abilities. Interestingly, the poly L-lactic acid nanofibrous scaffolds showed a significant support in recovering the osteogenic abilities of hMSCs, which had been severely affected by prolonged culture. © 2011 Future Medicine Ltd.
Source Title: Nanomedicine
ISSN: 17435889
DOI: 10.2217/nnm.11.26
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jul 10, 2020


checked on Jul 10, 2020

Page view(s)

checked on Jun 29, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.